Mouse skin passage of Streptococcus pyogenes results in increased streptokinase expression and activity
The plasminogen activator streptokinase has been proposed to be a key component of a complex mechanism that promotes skin invasion by Streptococcus pyogenes. This study was designed to compare ska gene message and protein levels in wild-type M1 serotype isolate 1881 and a more invasive variant recovered from the spleen of a lethally infected mouse. M1 isolates selected for invasiveness demonstrated enhanced levels of active plasminogen activator activity in culture. This effect was due to a combination of increased expression of the ska gene and decreased expression of the speB gene. The speB gene product, SpeB, was found to efficiently degrade streptokinase in vitro.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2004 |
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| Erschienen: |
2004 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:150 |
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| Enthalten in: |
Microbiology (Reading, England) - 150(2004), Pt 2 vom: 23. Feb., Seite 365-371 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Rezcallah, Myrna S [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 09.04.2004 Date Revised 26.08.2020 published: Print Citation Status MEDLINE |
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| doi: |
10.1099/mic.0.26826-0 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM144713195 |
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| 100 | 1 | |a Rezcallah, Myrna S |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Mouse skin passage of Streptococcus pyogenes results in increased streptokinase expression and activity |
| 264 | 1 | |c 2004 | |
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| 500 | |a Date Completed 09.04.2004 | ||
| 500 | |a Date Revised 26.08.2020 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The plasminogen activator streptokinase has been proposed to be a key component of a complex mechanism that promotes skin invasion by Streptococcus pyogenes. This study was designed to compare ska gene message and protein levels in wild-type M1 serotype isolate 1881 and a more invasive variant recovered from the spleen of a lethally infected mouse. M1 isolates selected for invasiveness demonstrated enhanced levels of active plasminogen activator activity in culture. This effect was due to a combination of increased expression of the ska gene and decreased expression of the speB gene. The speB gene product, SpeB, was found to efficiently degrade streptokinase in vitro | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a Streptokinase |2 NLM | |
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| 650 | 7 | |a Cysteine Endopeptidases |2 NLM | |
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| 650 | 7 | |a streptopain |2 NLM | |
| 650 | 7 | |a EC 3.4.22.10 |2 NLM | |
| 700 | 1 | |a Boyle, Michael D P |e verfasserin |4 aut | |
| 700 | 1 | |a Sledjeski, Darren D |e verfasserin |4 aut | |
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