Comparison of the sensitivity of different toxicological endpoints in Caco-2 cells after cadmium chloride treatment
The human colorectal adenocarcinoma cell line Caco-2 is a widely used in vitro model of the intestinal barrier. Cadmium chloride (CdCl(2)) is a highly toxic metal compound, ubiquitous in the biosphere, able to enter the food chain and to reach the intestinal epithelium, causing structural and functional damages. The aim of this work was to characterise cadmium toxicity in Caco-2 cells and, in particular, to compare the sensitivity of different endpoints revealing damage both on the epithelial barrier and at the cellular or molecular level. After 24-h exposure of the cells to CdCl(2), lactate dehydrogenase (LDH) leakage showed cadmium-induced cell toxicity, significant from 25 microM CdCl(2) and above, and analysis of different cell death pathways indicated the presence of necrosis after treatment with 50 microM CdCl(2). At the molecular level, we observed an increase in the protective protein heat shock protein 70 (HSP70), starting at 10 microM CdCl(2). At the barrier level, transepithelial electrical resistance (TEER) decreased while paracellular permeability (PCP) significantly increased after the treatment, showing an EC(50) of 6 and 16 microM CdCl(2), respectively, and indicating the loss of barrier integrity. In conclusion, our data reveal that CdCl(2) toxicity in Caco-2 cells can be detected at the barrier level at very low concentrations; also, HSP70 was shown to be a sensitive marker for detecting in vitro cadmium-induced toxicity.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2004 |
|---|---|
| Erschienen: |
2004 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:78 |
|---|---|
| Enthalten in: |
Archives of toxicology - 78(2004), 4 vom: 18. Apr., Seite 201-6 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Boveri, Monica [VerfasserIn] |
|---|
| Themen: |
ATP Binding Cassette Transporter, Subfamily B, Member 1 |
|---|
| Anmerkungen: |
Date Completed 02.12.2004 Date Revised 30.11.2018 published: Print-Electronic Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM143755919 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM143755919 | ||
| 003 | DE-627 | ||
| 005 | 20231223031615.0 | ||
| 007 | tu | ||
| 008 | 231223s2004 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0479.xml |
| 035 | |a (DE-627)NLM143755919 | ||
| 035 | |a (NLM)14661071 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Boveri, Monica |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Comparison of the sensitivity of different toxicological endpoints in Caco-2 cells after cadmium chloride treatment |
| 264 | 1 | |c 2004 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 02.12.2004 | ||
| 500 | |a Date Revised 30.11.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The human colorectal adenocarcinoma cell line Caco-2 is a widely used in vitro model of the intestinal barrier. Cadmium chloride (CdCl(2)) is a highly toxic metal compound, ubiquitous in the biosphere, able to enter the food chain and to reach the intestinal epithelium, causing structural and functional damages. The aim of this work was to characterise cadmium toxicity in Caco-2 cells and, in particular, to compare the sensitivity of different endpoints revealing damage both on the epithelial barrier and at the cellular or molecular level. After 24-h exposure of the cells to CdCl(2), lactate dehydrogenase (LDH) leakage showed cadmium-induced cell toxicity, significant from 25 microM CdCl(2) and above, and analysis of different cell death pathways indicated the presence of necrosis after treatment with 50 microM CdCl(2). At the molecular level, we observed an increase in the protective protein heat shock protein 70 (HSP70), starting at 10 microM CdCl(2). At the barrier level, transepithelial electrical resistance (TEER) decreased while paracellular permeability (PCP) significantly increased after the treatment, showing an EC(50) of 6 and 16 microM CdCl(2), respectively, and indicating the loss of barrier integrity. In conclusion, our data reveal that CdCl(2) toxicity in Caco-2 cells can be detected at the barrier level at very low concentrations; also, HSP70 was shown to be a sensitive marker for detecting in vitro cadmium-induced toxicity | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a ATP Binding Cassette Transporter, Subfamily B, Member 1 |2 NLM | |
| 650 | 7 | |a HSP70 Heat-Shock Proteins |2 NLM | |
| 650 | 7 | |a L-Lactate Dehydrogenase |2 NLM | |
| 650 | 7 | |a EC 1.1.1.27 |2 NLM | |
| 650 | 7 | |a Cadmium Chloride |2 NLM | |
| 650 | 7 | |a J6K4F9V3BA |2 NLM | |
| 700 | 1 | |a Pazos, Patricia |e verfasserin |4 aut | |
| 700 | 1 | |a Gennari, Alessandra |e verfasserin |4 aut | |
| 700 | 1 | |a Casado, Juan |e verfasserin |4 aut | |
| 700 | 1 | |a Hartung, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Prieto, Pilar |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Archives of toxicology |d 1974 |g 78(2004), 4 vom: 18. Apr., Seite 201-6 |w (DE-627)NLM000013374 |x 1432-0738 |7 nnns |
| 773 | 1 | 8 | |g volume:78 |g year:2004 |g number:4 |g day:18 |g month:04 |g pages:201-6 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 78 |j 2004 |e 4 |b 18 |c 04 |h 201-6 | ||