A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide
Peptide-oligonucleotide conjugates were synthesized using two strategies: a mimetic signal peptide-conjugated oligonucleotide was assembled stepwise on CPG by using 2,2-dimethyl-3-hydroxypropionic acid as a linker. To solve the precipitation problem in the coupling reaction caused by the electrostatic interaction of arginine-rich peptides and oligonucleotide, oligonucleotides were absorbed on an anion-exchange resin, and then the on-resin fragment was applied for the conjugation with arginine-rich peptide. The peptide-antisense oligonucleotides showed permeability to the cell membrane of HepG-2 cells.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2003 |
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| Erschienen: |
2003 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Bioconjugate chemistry - 14(2003), 3 vom: 20. Mai, Seite 532-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chen, Chang-Po [VerfasserIn] |
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| Themen: |
94ZLA3W45F |
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| Anmerkungen: |
Date Completed 16.01.2004 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM12524780X |
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| 100 | 1 | |a Chen, Chang-Po |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide |
| 264 | 1 | |c 2003 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 16.01.2004 | ||
| 500 | |a Date Revised 21.11.2013 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Peptide-oligonucleotide conjugates were synthesized using two strategies: a mimetic signal peptide-conjugated oligonucleotide was assembled stepwise on CPG by using 2,2-dimethyl-3-hydroxypropionic acid as a linker. To solve the precipitation problem in the coupling reaction caused by the electrostatic interaction of arginine-rich peptides and oligonucleotide, oligonucleotides were absorbed on an anion-exchange resin, and then the on-resin fragment was applied for the conjugation with arginine-rich peptide. The peptide-antisense oligonucleotides showed permeability to the cell membrane of HepG-2 cells | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Oligonucleotides, Antisense |2 NLM | |
| 650 | 7 | |a Peptides |2 NLM | |
| 650 | 7 | |a Arginine |2 NLM | |
| 650 | 7 | |a 94ZLA3W45F |2 NLM | |
| 700 | 1 | |a Zhang, Liang-Ren |e verfasserin |4 aut | |
| 700 | 1 | |a Peng, Yue-Feng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiao-Bo |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Sheng-Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Li-He |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Bioconjugate chemistry |d 1992 |g 14(2003), 3 vom: 20. Mai, Seite 532-8 |w (DE-627)NLM012617032 |x 1520-4812 |7 nnns |
| 773 | 1 | 8 | |g volume:14 |g year:2003 |g number:3 |g day:20 |g month:05 |g pages:532-8 |
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