Electroconvulsive shock increases serotonin transporter in the rat frontal cortex
The antidepressive action of electroconvulsive shock (ECS) is thought to involve the alteration in serotonin (5-HT) neurotransmission, including the increase in 5-HT release and uptake. In our previous study, 5-HT transporter (5-HTT) mRNA expression was decreased after single and repetitive ECS in rat raphe nucleus. In the present study, we investigated the effects of single and repetitive ECS on the protein levels of 5-HTT in the frontal cortex, hippocampus and raphe nucleus of rat brain using quantitative Western blot analysis. Single ECS did not alter 5-HTT protein expression in any brain regions examined. Repetitive ECS stably increased 5-HTT protein in the frontal cortex, but not in the hippocampus and raphe nucleus. Because ECS is known to facilitate the release of neurotransmitters, our results suggest that the increased 5-HTT protein expression in the frontal cortex might be a compensatory change against the enhanced 5-HT release by ECS in presynaptic terminals.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2003 |
|---|---|
| Erschienen: |
2003 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:341 |
|---|---|
| Enthalten in: |
Neuroscience letters - 341(2003), 2 vom: 01. Mai, Seite 170-2 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Shen, Hao wei [VerfasserIn] |
|---|
| Anmerkungen: |
Date Completed 15.05.2003 Date Revised 01.07.2019 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM12458151X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM12458151X | ||
| 003 | DE-627 | ||
| 005 | 20231222203843.0 | ||
| 007 | tu | ||
| 008 | 231222s2003 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0415.xml |
| 035 | |a (DE-627)NLM12458151X | ||
| 035 | |a (NLM)12686393 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Shen, Hao wei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Electroconvulsive shock increases serotonin transporter in the rat frontal cortex |
| 264 | 1 | |c 2003 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 15.05.2003 | ||
| 500 | |a Date Revised 01.07.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The antidepressive action of electroconvulsive shock (ECS) is thought to involve the alteration in serotonin (5-HT) neurotransmission, including the increase in 5-HT release and uptake. In our previous study, 5-HT transporter (5-HTT) mRNA expression was decreased after single and repetitive ECS in rat raphe nucleus. In the present study, we investigated the effects of single and repetitive ECS on the protein levels of 5-HTT in the frontal cortex, hippocampus and raphe nucleus of rat brain using quantitative Western blot analysis. Single ECS did not alter 5-HTT protein expression in any brain regions examined. Repetitive ECS stably increased 5-HTT protein in the frontal cortex, but not in the hippocampus and raphe nucleus. Because ECS is known to facilitate the release of neurotransmitters, our results suggest that the increased 5-HTT protein expression in the frontal cortex might be a compensatory change against the enhanced 5-HT release by ECS in presynaptic terminals | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Carrier Proteins |2 NLM | |
| 650 | 7 | |a DNA Primers |2 NLM | |
| 650 | 7 | |a Membrane Glycoproteins |2 NLM | |
| 650 | 7 | |a Membrane Transport Proteins |2 NLM | |
| 650 | 7 | |a Nerve Tissue Proteins |2 NLM | |
| 650 | 7 | |a Serotonin Plasma Membrane Transport Proteins |2 NLM | |
| 650 | 7 | |a Slc6a4 protein, rat |2 NLM | |
| 700 | 1 | |a Numachi, Yohtaro |e verfasserin |4 aut | |
| 700 | 1 | |a Yoshida, Sumiko |e verfasserin |4 aut | |
| 700 | 1 | |a Fujiyama, Ko |e verfasserin |4 aut | |
| 700 | 1 | |a Toda, Shigenobu |e verfasserin |4 aut | |
| 700 | 1 | |a Awata, Shuichi |e verfasserin |4 aut | |
| 700 | 1 | |a Matsuoka, Hiroo |e verfasserin |4 aut | |
| 700 | 1 | |a Sato, Mitsumoto |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Neuroscience letters |d 1975 |g 341(2003), 2 vom: 01. Mai, Seite 170-2 |w (DE-627)NLM000386529 |x 1872-7972 |7 nnns |
| 773 | 1 | 8 | |g volume:341 |g year:2003 |g number:2 |g day:01 |g month:05 |g pages:170-2 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 341 |j 2003 |e 2 |b 01 |c 05 |h 170-2 | ||