Rapid diagnosis of cytomegalovirus in Thai pediatric AIDS patients

Blood samples were collected from 100 pediatric AIDS patients for the detection of CMV in pp65-bearing leukocytes (PBLs) by immunoperoxidase staining (IP) and PCR. IgM antibody assay was performed to determine the correlation of antigen and antibody. IP and PCR can be used as methods for the early detection of CMV (prior to the presence of IgM antibody). The sensitivity and specificity of IP were 73% and 97% respectively. IP is superior to PCR in several ways: it is very easy to perform, less time consuming, less expensive, and does not require expensive instruments.

Medienart:

Artikel

Erscheinungsjahr:

2002

Erschienen:

2002

Enthalten in:

Zur Gesamtaufnahme - volume:33

Enthalten in:

The Southeast Asian journal of tropical medicine and public health - 33(2002), 2 vom: 01. Juni, Seite 306-11

Sprache:

Englisch

Beteiligte Personen:

Tantivanich, Surang [VerfasserIn]
Sawatmongkonkun, Wandee [VerfasserIn]
Balachandra, Kruavan [VerfasserIn]
Chotpitayasunondh, Tawee [VerfasserIn]
Amarapal, Pomsawan [VerfasserIn]

Themen:

DNA Primers
Journal Article
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 27.01.2003

Date Revised 15.11.2006

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM120935821