Evidence for a quinone binding site close to the interface between NUOD and NUOB subunits of Complex I
Piericidin, rotenone and pyridaben are specific inhibitors of the NADH-ubiquinone oxidoreductase (Complex I) that bind to its ubiquinone binding site(s). Using site directed mutagenesis, we demonstrate that residues G409, D412, R413 and V407 of the C-terminus of Complex I NUOD subunit are directly involved in the binding of these inhibitors. We propose that the corresponding inhibitor/quinone binding site would be located close to NUOD-NUOB interface.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2001 |
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| Erschienen: |
2001 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1504 |
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| Enthalten in: |
Biochimica et biophysica acta - 1504(2001), 2-3 vom: 02. Apr., Seite 173-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Prieur, I [VerfasserIn] |
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| Anmerkungen: |
Date Completed 26.04.2001 Date Revised 10.12.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM11156655X |
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| 100 | 1 | |a Prieur, I |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Evidence for a quinone binding site close to the interface between NUOD and NUOB subunits of Complex I |
| 264 | 1 | |c 2001 | |
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| 500 | |a Date Completed 26.04.2001 | ||
| 500 | |a Date Revised 10.12.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Piericidin, rotenone and pyridaben are specific inhibitors of the NADH-ubiquinone oxidoreductase (Complex I) that bind to its ubiquinone binding site(s). Using site directed mutagenesis, we demonstrate that residues G409, D412, R413 and V407 of the C-terminus of Complex I NUOD subunit are directly involved in the binding of these inhibitors. We propose that the corresponding inhibitor/quinone binding site would be located close to NUOD-NUOB interface | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Enzyme Inhibitors |2 NLM | |
| 650 | 7 | |a Photoaffinity Labels |2 NLM | |
| 650 | 7 | |a Pyridines |2 NLM | |
| 650 | 7 | |a Quinones |2 NLM | |
| 650 | 7 | |a Rotenone |2 NLM | |
| 650 | 7 | |a 03L9OT429T |2 NLM | |
| 650 | 7 | |a piericidin A |2 NLM | |
| 650 | 7 | |a 8VT513UJ9R |2 NLM | |
| 650 | 7 | |a NADH, NADPH Oxidoreductases |2 NLM | |
| 650 | 7 | |a EC 1.6.- |2 NLM | |
| 650 | 7 | |a Electron Transport Complex I |2 NLM | |
| 650 | 7 | |a EC 7.1.1.2 |2 NLM | |
| 700 | 1 | |a Lunardi, J |e verfasserin |4 aut | |
| 700 | 1 | |a Dupuis, A |e verfasserin |4 aut | |
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