CD40 and adenosine A2 receptor agonist-cyclic adenosine monophosphate rescue B-cell antigen receptor-induced apoptosis through independent pathways and converge to prevent caspase activation
BACKGROUND: Antigen receptor ligation induces apoptosis of B lymphocytes, but the molecular mechanisms underlying induction of apoptosis remain unclear, although the growing family of IL-1beta-converting enzyme cysteine proteases (caspases) are recognized to be major effectors of cellular death.
OBJECTIVE: We sought to delineate and compare the rescue of B-cell apoptosis through CD40 ligand-CD40 interaction and cyclic adenosine monophosphate (cAMP)-dependent protein kinase A in human B cells.
METHODS: By using tonsillar B cells and the B-lymphoblastoid cell line Ramos, rescue from B-cell apoptosis was compared, as were signaling pathways after activation of cells through CD40 and the adenosine A2 receptor.
RESULTS: Both CD40 ligand-CD40 interaction and activation of intracellular cAMP rescue B cells from apoptosis after antigen receptor ligation. Although these pathways do not overlap, they converge by preventing the anti-IgM-induced activation of CPP32 (caspase 3), a member of the IL-1beta-converting enzyme protease family.
CONCLUSION: These data indicate that the cAMP-protein kinase A-dependent and CD40-signaling pathways regulate B-cell survival and converge at a common point, the inhibition of antigen receptor-induced activation of caspases.
Medienart: |
Artikel |
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Erscheinungsjahr: |
2000 |
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Erschienen: |
2000 |
Enthalten in: |
Zur Gesamtaufnahme - volume:105 |
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Enthalten in: |
The Journal of allergy and clinical immunology - 105(2000), 3 vom: 15. März, Seite 522-31 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Sakata, N [VerfasserIn] |
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Anmerkungen: |
Date Completed 13.04.2000 Date Revised 16.11.2017 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM106469037 |
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100 | 1 | |a Sakata, N |e verfasserin |4 aut | |
245 | 1 | 0 | |a CD40 and adenosine A2 receptor agonist-cyclic adenosine monophosphate rescue B-cell antigen receptor-induced apoptosis through independent pathways and converge to prevent caspase activation |
264 | 1 | |c 2000 | |
336 | |a Text |b txt |2 rdacontent | ||
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500 | |a Date Completed 13.04.2000 | ||
500 | |a Date Revised 16.11.2017 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a BACKGROUND: Antigen receptor ligation induces apoptosis of B lymphocytes, but the molecular mechanisms underlying induction of apoptosis remain unclear, although the growing family of IL-1beta-converting enzyme cysteine proteases (caspases) are recognized to be major effectors of cellular death | ||
520 | |a OBJECTIVE: We sought to delineate and compare the rescue of B-cell apoptosis through CD40 ligand-CD40 interaction and cyclic adenosine monophosphate (cAMP)-dependent protein kinase A in human B cells | ||
520 | |a METHODS: By using tonsillar B cells and the B-lymphoblastoid cell line Ramos, rescue from B-cell apoptosis was compared, as were signaling pathways after activation of cells through CD40 and the adenosine A2 receptor | ||
520 | |a RESULTS: Both CD40 ligand-CD40 interaction and activation of intracellular cAMP rescue B cells from apoptosis after antigen receptor ligation. Although these pathways do not overlap, they converge by preventing the anti-IgM-induced activation of CPP32 (caspase 3), a member of the IL-1beta-converting enzyme protease family | ||
520 | |a CONCLUSION: These data indicate that the cAMP-protein kinase A-dependent and CD40-signaling pathways regulate B-cell survival and converge at a common point, the inhibition of antigen receptor-induced activation of caspases | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
650 | 7 | |a Antibodies |2 NLM | |
650 | 7 | |a Antibodies, Anti-Idiotypic |2 NLM | |
650 | 7 | |a CD40 Antigens |2 NLM | |
650 | 7 | |a Cysteine Proteinase Inhibitors |2 NLM | |
650 | 7 | |a Proto-Oncogene Proteins c-bcl-2 |2 NLM | |
650 | 7 | |a Purinergic P1 Receptor Agonists |2 NLM | |
650 | 7 | |a Receptors, Antigen, B-Cell |2 NLM | |
650 | 7 | |a anti-IgM |2 NLM | |
650 | 7 | |a Bucladesine |2 NLM | |
650 | 7 | |a 63X7MBT2LQ |2 NLM | |
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700 | 1 | |a Terada, N |e verfasserin |4 aut | |
700 | 1 | |a Johnson, G L |e verfasserin |4 aut | |
700 | 1 | |a Gelfand, E W |e verfasserin |4 aut | |
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