Early damage of sympathetic neurons after co-culture with macrophages : a model of neuronal injury in vitro
Since activated immune cells may damage peripheral nerves during inflammation, we developed a co-culture model that permits the direct study of macrophage-induced neuronal damage. Sympathetic neurons were enzymatically isolated from neonatal mice and co-cultured with increasing numbers of peritoneal macrophages for 24 h. This caused rapid neuronal cell death, reducing neuronal number by 24.1 +/- 4% with the addition of 11.5 x 10(3) macrophages, representing a ratio of 8 macrophages per neuron. Nuclear analysis showed that cell death occurred by both apoptosis and necrosis. These effects were not mimicked by addition of macrophage-conditioned medium, and were prevented by 10 microM dexamethasone. Although no appreciable neuronal death occurred beyond 24 h, the density of neurites was decreased between 1 and 2 days of co-culture (p < 0.05). There is, therefore, a rapid induction of cytotoxicity by macrophages after their addition to the neuronal cultures, followed by axonal damage without neuronal cell death.
| Errataetall: | |
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| Medienart: |
Artikel |
| Erscheinungsjahr: |
2000 |
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| Erschienen: |
2000 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Neuroreport - 11(2000), 1 vom: 17. Jan., Seite 177-81 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Arantes, R M [VerfasserIn] |
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| Themen: |
7S5I7G3JQL |
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| Anmerkungen: |
Date Completed 16.03.2000 Date Revised 18.07.2019 published: Print ErratumIn: Neuroreport 2000 Apr 7;11(5):inside back cov Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM106118684 |
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| 245 | 1 | 0 | |a Early damage of sympathetic neurons after co-culture with macrophages |b a model of neuronal injury in vitro |
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| 500 | |a Date Completed 16.03.2000 | ||
| 500 | |a Date Revised 18.07.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a ErratumIn: Neuroreport 2000 Apr 7;11(5):inside back cov | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Since activated immune cells may damage peripheral nerves during inflammation, we developed a co-culture model that permits the direct study of macrophage-induced neuronal damage. Sympathetic neurons were enzymatically isolated from neonatal mice and co-cultured with increasing numbers of peritoneal macrophages for 24 h. This caused rapid neuronal cell death, reducing neuronal number by 24.1 +/- 4% with the addition of 11.5 x 10(3) macrophages, representing a ratio of 8 macrophages per neuron. Nuclear analysis showed that cell death occurred by both apoptosis and necrosis. These effects were not mimicked by addition of macrophage-conditioned medium, and were prevented by 10 microM dexamethasone. Although no appreciable neuronal death occurred beyond 24 h, the density of neurites was decreased between 1 and 2 days of co-culture (p < 0.05). There is, therefore, a rapid induction of cytotoxicity by macrophages after their addition to the neuronal cultures, followed by axonal damage without neuronal cell death | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Blennerhassett, M G |e verfasserin |4 aut | |
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