Non-viral peptide-based approaches to gene delivery
To achieve effective non-viral gene therapy, the control of in vitro and in vivo stability, cellular access, intracellular trafficking and nuclear retention of plasmids must be achieved. Inefficient endosomal release, stability against cytosolic nucleases, cytoplasmic transport and nuclear entry of plasmids are amongst some of the key limiting factors in the use of plasmids for effective gene therapy. Synthetic peptide-based gene delivery systems can be designed for DNA compaction, serum stability, cell-specific targeting, endosomolysis, cytoplasmic stability and nuclear transport. The stability of compacted DNA under physiological conditions can be enhanced by the use of hydrophilic polymers, such as polyethylene glycol. The aims of this review are to (i) explore theoretical and experimental aspects of DNA compaction, (ii) describe approaches for stabilizing compacted DNA, (iii) assess techniques used for characterization of compacted DNA, and (iv) review possible use of peptides for efficient gene transfer.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
1999 |
|---|---|
| Erschienen: |
1999 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
|---|---|
| Enthalten in: |
Journal of drug targeting - 7(1999), 4 vom: 28. Dez., Seite 249-68 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Mahato, R I [VerfasserIn] |
|---|
| Themen: |
|---|
| Anmerkungen: |
Date Completed 17.03.2000 Date Revised 15.11.2012 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM106109243 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM106109243 | ||
| 003 | DE-627 | ||
| 005 | 20231222140126.0 | ||
| 007 | tu | ||
| 008 | 231222s1999 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0354.xml |
| 035 | |a (DE-627)NLM106109243 | ||
| 035 | |a (NLM)10682905 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Mahato, R I |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Non-viral peptide-based approaches to gene delivery |
| 264 | 1 | |c 1999 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 17.03.2000 | ||
| 500 | |a Date Revised 15.11.2012 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a To achieve effective non-viral gene therapy, the control of in vitro and in vivo stability, cellular access, intracellular trafficking and nuclear retention of plasmids must be achieved. Inefficient endosomal release, stability against cytosolic nucleases, cytoplasmic transport and nuclear entry of plasmids are amongst some of the key limiting factors in the use of plasmids for effective gene therapy. Synthetic peptide-based gene delivery systems can be designed for DNA compaction, serum stability, cell-specific targeting, endosomolysis, cytoplasmic stability and nuclear transport. The stability of compacted DNA under physiological conditions can be enhanced by the use of hydrophilic polymers, such as polyethylene glycol. The aims of this review are to (i) explore theoretical and experimental aspects of DNA compaction, (ii) describe approaches for stabilizing compacted DNA, (iii) assess techniques used for characterization of compacted DNA, and (iv) review possible use of peptides for efficient gene transfer | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Review | |
| 650 | 7 | |a Peptides |2 NLM | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of drug targeting |d 1996 |g 7(1999), 4 vom: 28. Dez., Seite 249-68 |w (DE-627)NLM074733958 |x 1029-2330 |7 nnns |
| 773 | 1 | 8 | |g volume:7 |g year:1999 |g number:4 |g day:28 |g month:12 |g pages:249-68 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 7 |j 1999 |e 4 |b 28 |c 12 |h 249-68 | ||