Analysis of 4-ABP-DNA adducts and p53 alterations in urinary bladder carcinoma
BACKGROUND: Activated intermediates of 4-aminobiphenyl (4-ABP) are able to covalently interact with DNA to form adducts. There is a large body of evidence indicating that carcinogen-DNA adduct formation can be one of the cancer initiating mechanisms.
MATERIALS AND METHODS: (4-ABP)-induced DNA damage in association with p53 overexpression and mutations were evaluated in specimens of urothelial bladder cancers from 106 patients.
RESULTS: 4-ABP-DNA adduct levels resulted higher in smokers compared to non smokers, with a borderline statistical value. p53 nuclear overexpression was related to tumor grading, while no significant correlation with stage, 4-ABP-DNA adducts, smoking habit, and disease recurrence could be observed. Concerning molecular analysis, p53 point mutations were found in 17 of 106 cases (16%) and mutational pattern was significantly associated both with higher grade and stage, but no correlation was found with disease recurrence.
CONCLUSIONS: These results suggest that other sources, in addition to tobacco smoke, may contribute to 4-ABP-DNA adducts formation in bladder tissue and that p53 expression/mutation cannot be considered a prognostic factor in bladder cancer.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1999 |
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| Erschienen: |
1999 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:19 |
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| Enthalten in: |
Anticancer research - 19(1999), 5C vom: 14. Sept., Seite 4571-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Romano, G [VerfasserIn] |
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| Themen: |
16054949HJ |
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| Anmerkungen: |
Date Completed 22.02.2000 Date Revised 15.11.2012 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM10579578X |
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| 245 | 1 | 0 | |a Analysis of 4-ABP-DNA adducts and p53 alterations in urinary bladder carcinoma |
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| 500 | |a Date Completed 22.02.2000 | ||
| 500 | |a Date Revised 15.11.2012 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: Activated intermediates of 4-aminobiphenyl (4-ABP) are able to covalently interact with DNA to form adducts. There is a large body of evidence indicating that carcinogen-DNA adduct formation can be one of the cancer initiating mechanisms | ||
| 520 | |a MATERIALS AND METHODS: (4-ABP)-induced DNA damage in association with p53 overexpression and mutations were evaluated in specimens of urothelial bladder cancers from 106 patients | ||
| 520 | |a RESULTS: 4-ABP-DNA adduct levels resulted higher in smokers compared to non smokers, with a borderline statistical value. p53 nuclear overexpression was related to tumor grading, while no significant correlation with stage, 4-ABP-DNA adducts, smoking habit, and disease recurrence could be observed. Concerning molecular analysis, p53 point mutations were found in 17 of 106 cases (16%) and mutational pattern was significantly associated both with higher grade and stage, but no correlation was found with disease recurrence | ||
| 520 | |a CONCLUSIONS: These results suggest that other sources, in addition to tobacco smoke, may contribute to 4-ABP-DNA adducts formation in bladder tissue and that p53 expression/mutation cannot be considered a prognostic factor in bladder cancer | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Aminobiphenyl Compounds |2 NLM | |
| 650 | 7 | |a Carcinogens |2 NLM | |
| 650 | 7 | |a DNA Adducts |2 NLM | |
| 650 | 7 | |a DNA, Neoplasm |2 NLM | |
| 650 | 7 | |a Tumor Suppressor Protein p53 |2 NLM | |
| 650 | 7 | |a 4-biphenylamine |2 NLM | |
| 650 | 7 | |a 16054949HJ |2 NLM | |
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| 700 | 1 | |a Flamini, G |e verfasserin |4 aut | |
| 700 | 1 | |a De Gaetani, C |e verfasserin |4 aut | |
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| 700 | 1 | |a Ferretti, G |e verfasserin |4 aut | |
| 700 | 1 | |a Cittadini, A |e verfasserin |4 aut | |
| 700 | 1 | |a Trentini, G |e verfasserin |4 aut | |
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