Combined effects of trehalose and cations on the thermal resistance of beta-galactosidase in freeze-dried systems
The purpose of this study was to investigate the combined effects of trehalose and cations on the preservation of beta-galactosidase in freeze-dried systems and their relationship to physical properties. Differential scanning calorimetry was employed to measure the glass transition temperature (T(g)) and the endothermal peak area, related to the amount of crystalline trehalose dihydrate present in the samples. In systems in which the trehalose matrix was humidified to conditions which allowed a high proportion of trehalose to crystallize, the enzyme was rapidly inactivated upon heating at 70 degrees C. In these conditions the addition of CsCl, NaCl and particularly KCl or MgCl(2), improved the enzyme stability with respect to that observed in matrices containing only trehalose. For a given moisture content, addition of salts produced very little change on the glass transition temperature; therefore the protective effect could not be attributed to a higher T(g) value. The crystallization of trehalose dihydrate in the humidified samples was delayed in the trehalose/salt systems (principally in the presence of Mg(2+)) and a parallel improvement of enzyme stability was observed.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
1999 |
|---|---|
| Erschienen: |
1999 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1473 |
|---|---|
| Enthalten in: |
Biochimica et biophysica acta - 1473(1999), 2-3 vom: 27. Dez., Seite 337-44 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Mazzobre, M F [VerfasserIn] |
|---|
| Anmerkungen: |
Date Completed 14.02.2000 Date Revised 10.06.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM105241660 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
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| 003 | DE-627 | ||
| 005 | 20231222134237.0 | ||
| 007 | tu | ||
| 008 | 231222s1999 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0351.xml |
| 035 | |a (DE-627)NLM105241660 | ||
| 035 | |a (NLM)10594371 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Mazzobre, M F |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Combined effects of trehalose and cations on the thermal resistance of beta-galactosidase in freeze-dried systems |
| 264 | 1 | |c 1999 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 14.02.2000 | ||
| 500 | |a Date Revised 10.06.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The purpose of this study was to investigate the combined effects of trehalose and cations on the preservation of beta-galactosidase in freeze-dried systems and their relationship to physical properties. Differential scanning calorimetry was employed to measure the glass transition temperature (T(g)) and the endothermal peak area, related to the amount of crystalline trehalose dihydrate present in the samples. In systems in which the trehalose matrix was humidified to conditions which allowed a high proportion of trehalose to crystallize, the enzyme was rapidly inactivated upon heating at 70 degrees C. In these conditions the addition of CsCl, NaCl and particularly KCl or MgCl(2), improved the enzyme stability with respect to that observed in matrices containing only trehalose. For a given moisture content, addition of salts produced very little change on the glass transition temperature; therefore the protective effect could not be attributed to a higher T(g) value. The crystallization of trehalose dihydrate in the humidified samples was delayed in the trehalose/salt systems (principally in the presence of Mg(2+)) and a parallel improvement of enzyme stability was observed | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Chlorides |2 NLM | |
| 650 | 7 | |a Magnesium Chloride |2 NLM | |
| 650 | 7 | |a 02F3473H9O |2 NLM | |
| 650 | 7 | |a Cesium |2 NLM | |
| 650 | 7 | |a 1KSV9V4Y4I |2 NLM | |
| 650 | 7 | |a Sodium Chloride |2 NLM | |
| 650 | 7 | |a 451W47IQ8X |2 NLM | |
| 650 | 7 | |a Potassium Chloride |2 NLM | |
| 650 | 7 | |a 660YQ98I10 |2 NLM | |
| 650 | 7 | |a Trehalose |2 NLM | |
| 650 | 7 | |a B8WCK70T7I |2 NLM | |
| 650 | 7 | |a Lactase |2 NLM | |
| 650 | 7 | |a EC 3.2.1.108 |2 NLM | |
| 650 | 7 | |a beta-Galactosidase |2 NLM | |
| 650 | 7 | |a EC 3.2.1.23 |2 NLM | |
| 650 | 7 | |a cesium chloride |2 NLM | |
| 650 | 7 | |a GNR9HML8BA |2 NLM | |
| 700 | 1 | |a Del Pilar Buera, M |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biochimica et biophysica acta |d 1948 |g 1473(1999), 2-3 vom: 27. Dez., Seite 337-44 |w (DE-627)NLM000000698 |x 0006-3002 |7 nnns |
| 773 | 1 | 8 | |g volume:1473 |g year:1999 |g number:2-3 |g day:27 |g month:12 |g pages:337-44 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 1473 |j 1999 |e 2-3 |b 27 |c 12 |h 337-44 | ||