Inhibitory effect of phosphate on in vitro development of 2-cell rat embryos is overcome by a factor(s) in oviductal extracts
It has been found that inorganic phosphate (P) at concentrations as low as 10 microM markedly inhibits in vitro development of early rat embryos at the 1-cell or 2-cell stage to the blastocyst stage, although P is present at concentrations of 0.37-1.19 mM in oviductal fluid, in which the development of early embryos occurs. We show here that fractions (PTF, 50-250 microg/ml) of rat oviductal extracts (OVEs) passed through a Blue CL6B affinity column have the ability to overcome the inhibitory effects of P on the development of 2-cell rat embryos in a dose-dependent manner, whereas 250 microg/ml OVE or 250 microg/ml of the bound fractions (BF) induced degenerative changes in the embryos at the 2-cell stage. Moreover, PTF at concentrations of >/=100 microg/ml stimulated the hatching of blastocysts both in medium with and without P, although none of the blastocysts in medium without PTF hatched from their zona pellucida.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1999 |
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| Erschienen: |
1999 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:462 |
|---|---|
| Enthalten in: |
FEBS letters - 462(1999), 1-2 vom: 26. Nov., Seite 71-4 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yamada, M [VerfasserIn] |
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| Themen: |
Biological Factors |
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| Anmerkungen: |
Date Completed 13.01.2000 Date Revised 21.06.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM105100374 |
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| 100 | 1 | |a Yamada, M |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Inhibitory effect of phosphate on in vitro development of 2-cell rat embryos is overcome by a factor(s) in oviductal extracts |
| 264 | 1 | |c 1999 | |
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| 500 | |a Date Completed 13.01.2000 | ||
| 500 | |a Date Revised 21.06.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a It has been found that inorganic phosphate (P) at concentrations as low as 10 microM markedly inhibits in vitro development of early rat embryos at the 1-cell or 2-cell stage to the blastocyst stage, although P is present at concentrations of 0.37-1.19 mM in oviductal fluid, in which the development of early embryos occurs. We show here that fractions (PTF, 50-250 microg/ml) of rat oviductal extracts (OVEs) passed through a Blue CL6B affinity column have the ability to overcome the inhibitory effects of P on the development of 2-cell rat embryos in a dose-dependent manner, whereas 250 microg/ml OVE or 250 microg/ml of the bound fractions (BF) induced degenerative changes in the embryos at the 2-cell stage. Moreover, PTF at concentrations of >/=100 microg/ml stimulated the hatching of blastocysts both in medium with and without P, although none of the blastocysts in medium without PTF hatched from their zona pellucida | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Biological Factors |2 NLM | |
| 650 | 7 | |a Organophosphates |2 NLM | |
| 700 | 1 | |a Nishikimi, A |e verfasserin |4 aut | |
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