Reverse transcriptase polymerase chain reaction-based diagnosis and molecular characterization of a new rat coronavirus strain
BACKGROUND AND PURPOSE: Rat coronaviruses (RCV) are highly infectious and spread rapidly through laboratory rat colonies, causing sneezing, nasal and ocular discharges, photophobia, and cervical swelling. Current diagnostic methods include serologic testing and histologic examination. During a recent rat coronavirus outbreak, we tested a rapid, noninvasive method of RCV diagnosis that involved use of reverse transcriptase-polymerase chain reaction (RT-PCR) analysis to detect RCV RNA on cages housing infected rats.
METHODS: The RT-PCR was used to detect RCV RNA in tissues from infected rats and on cages housing infected rats and to amplify portions of the RCV N, M, and S genes for molecular characterization.
RESULTS: The RT-PCR detected RCV RNA on cages and in tissues from infected rats. The RCV-NJ N gene is most closely related to the MHV-Y N gene. The M proteins of RCV-NJ and RCV-SDA are 99% homologous, and the six RCV S protein fragments are 97 to 100% homologous.
CONCLUSIONS: Use of RT-PCR with cage-swab specimens was capable of diagnosing RCV infection in and viral excretion from rats. Additionally, molecular characterization of the N, M, and S genes of RCV-NJ provided baseline information that can be used in performing further epidemiologic studies.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
1999 |
|---|---|
| Erschienen: |
1999 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:49 |
|---|---|
| Enthalten in: |
Laboratory animal science - 49(1999), 5 vom: 31. Okt., Seite 506-13 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Compton, S R [VerfasserIn] |
|---|
| Anmerkungen: |
Date Completed 23.12.1999 Date Revised 08.12.2020 published: Print GENBANK: AF088984, AF088985, AF088986, AF088987, AF088988, AF088989, AF088990 Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM104817925 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM104817925 | ||
| 003 | DE-627 | ||
| 005 | 20231222133332.0 | ||
| 007 | tu | ||
| 008 | 231222s1999 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0350.xml |
| 035 | |a (DE-627)NLM104817925 | ||
| 035 | |a (NLM)10551451 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Compton, S R |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Reverse transcriptase polymerase chain reaction-based diagnosis and molecular characterization of a new rat coronavirus strain |
| 264 | 1 | |c 1999 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 23.12.1999 | ||
| 500 | |a Date Revised 08.12.2020 | ||
| 500 | |a published: Print | ||
| 500 | |a GENBANK: AF088984, AF088985, AF088986, AF088987, AF088988, AF088989, AF088990 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND AND PURPOSE: Rat coronaviruses (RCV) are highly infectious and spread rapidly through laboratory rat colonies, causing sneezing, nasal and ocular discharges, photophobia, and cervical swelling. Current diagnostic methods include serologic testing and histologic examination. During a recent rat coronavirus outbreak, we tested a rapid, noninvasive method of RCV diagnosis that involved use of reverse transcriptase-polymerase chain reaction (RT-PCR) analysis to detect RCV RNA on cages housing infected rats | ||
| 520 | |a METHODS: The RT-PCR was used to detect RCV RNA in tissues from infected rats and on cages housing infected rats and to amplify portions of the RCV N, M, and S genes for molecular characterization | ||
| 520 | |a RESULTS: The RT-PCR detected RCV RNA on cages and in tissues from infected rats. The RCV-NJ N gene is most closely related to the MHV-Y N gene. The M proteins of RCV-NJ and RCV-SDA are 99% homologous, and the six RCV S protein fragments are 97 to 100% homologous | ||
| 520 | |a CONCLUSIONS: Use of RT-PCR with cage-swab specimens was capable of diagnosing RCV infection in and viral excretion from rats. Additionally, molecular characterization of the N, M, and S genes of RCV-NJ provided baseline information that can be used in performing further epidemiologic studies | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Coronavirus M Proteins |2 NLM | |
| 650 | 7 | |a Coronavirus Nucleocapsid Proteins |2 NLM | |
| 650 | 7 | |a Nucleocapsid Proteins |2 NLM | |
| 650 | 7 | |a Plastics |2 NLM | |
| 650 | 7 | |a RNA, Viral |2 NLM | |
| 650 | 7 | |a Viral Matrix Proteins |2 NLM | |
| 650 | 7 | |a Viral Proteins |2 NLM | |
| 650 | 7 | |a DNA Restriction Enzymes |2 NLM | |
| 650 | 7 | |a EC 3.1.21.- |2 NLM | |
| 700 | 1 | |a Vivas-Gonzalez, B E |e verfasserin |4 aut | |
| 700 | 1 | |a Macy, J D |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Laboratory animal science |d 1971 |g 49(1999), 5 vom: 31. Okt., Seite 506-13 |w (DE-627)NLM000062480 |x 0023-6764 |7 nnns |
| 773 | 1 | 8 | |g volume:49 |g year:1999 |g number:5 |g day:31 |g month:10 |g pages:506-13 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 49 |j 1999 |e 5 |b 31 |c 10 |h 506-13 | ||