Transforming growth factor-beta1 modulates the expression of vascular endothelial growth factor by osteoblasts
Angiogenesis is essential to both normal and pathological bone physiology. Vascular endothelial growth factor (VEGF) has been implicated in angiogenesis, whereas transforming growth factor-beta1 (TGF-beta1) modulates bone differentiation, matrix formation, and cytokine expression. The purpose of this study was to investigate the relationship between TGF-beta1 and VEGF expression in osteoblasts and osteoblast-like cells. Northern blot analysis revealed an early peak of VEGF mRNA (6-fold at 3 h) in fetal rat calvarial cells and MC3T3-E1 osteoblast-like cells after stimulation with TGF-beta1 (2.5 ng/ml). The stability of VEGF mRNA in MC3T3-E1 cells was not increased after TGF-beta1 treatment. Actinomycin D inhibited the TGF-beta1-induced peak in VEGF mRNA, whereas cycloheximide did not. Blockade of TGF-beta1 signal transduction via a dominant-negative receptor II adenovirus significantly decreased TGF-beta1 induction of VEGF mRNA. Additionally, TGF-beta1 induced a dose-dependent increase in VEGF protein expression by MC3T3-E1 cells (P < 0.01). Dexamethasone similarly inhibited VEGF protein expression. Both TGF-beta1 mRNA and VEGF mRNA were concurrently present in rat membranous bone, and both followed similar patterns of expression during rat mandibular fracture healing (mRNA and protein). In summary, TGF-beta1-induced VEGF expression by osteoblasts and osteoblast-like cells is a dose-dependent event that may be intimately related to bone development and fracture healing.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
1999 |
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| Erschienen: |
1999 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:277 |
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| Enthalten in: |
The American journal of physiology - 277(1999), 4 vom: 15. Okt., Seite C628-37 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Saadeh, P B [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 22.11.1999 Date Revised 05.10.2018 published: Print Citation Status MEDLINE |
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| doi: |
10.1152/ajpcell.1999.277.4.C628 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM104468424 |
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| 100 | 1 | |a Saadeh, P B |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Transforming growth factor-beta1 modulates the expression of vascular endothelial growth factor by osteoblasts |
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| 500 | |a Date Completed 22.11.1999 | ||
| 500 | |a Date Revised 05.10.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Angiogenesis is essential to both normal and pathological bone physiology. Vascular endothelial growth factor (VEGF) has been implicated in angiogenesis, whereas transforming growth factor-beta1 (TGF-beta1) modulates bone differentiation, matrix formation, and cytokine expression. The purpose of this study was to investigate the relationship between TGF-beta1 and VEGF expression in osteoblasts and osteoblast-like cells. Northern blot analysis revealed an early peak of VEGF mRNA (6-fold at 3 h) in fetal rat calvarial cells and MC3T3-E1 osteoblast-like cells after stimulation with TGF-beta1 (2.5 ng/ml). The stability of VEGF mRNA in MC3T3-E1 cells was not increased after TGF-beta1 treatment. Actinomycin D inhibited the TGF-beta1-induced peak in VEGF mRNA, whereas cycloheximide did not. Blockade of TGF-beta1 signal transduction via a dominant-negative receptor II adenovirus significantly decreased TGF-beta1 induction of VEGF mRNA. Additionally, TGF-beta1 induced a dose-dependent increase in VEGF protein expression by MC3T3-E1 cells (P < 0.01). Dexamethasone similarly inhibited VEGF protein expression. Both TGF-beta1 mRNA and VEGF mRNA were concurrently present in rat membranous bone, and both followed similar patterns of expression during rat mandibular fracture healing (mRNA and protein). In summary, TGF-beta1-induced VEGF expression by osteoblasts and osteoblast-like cells is a dose-dependent event that may be intimately related to bone development and fracture healing | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Culture Media, Conditioned |2 NLM | |
| 650 | 7 | |a Endothelial Growth Factors |2 NLM | |
| 650 | 7 | |a Glucocorticoids |2 NLM | |
| 650 | 7 | |a Lymphokines |2 NLM | |
| 650 | 7 | |a Protein Synthesis Inhibitors |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a Transforming Growth Factor beta |2 NLM | |
| 650 | 7 | |a Vascular Endothelial Growth Factor A |2 NLM | |
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| 700 | 1 | |a Mehrara, B J |e verfasserin |4 aut | |
| 700 | 1 | |a Steinbrech, D S |e verfasserin |4 aut | |
| 700 | 1 | |a Dudziak, M E |e verfasserin |4 aut | |
| 700 | 1 | |a Greenwald, J A |e verfasserin |4 aut | |
| 700 | 1 | |a Luchs, J S |e verfasserin |4 aut | |
| 700 | 1 | |a Spector, J A |e verfasserin |4 aut | |
| 700 | 1 | |a Ueno, H |e verfasserin |4 aut | |
| 700 | 1 | |a Gittes, G K |e verfasserin |4 aut | |
| 700 | 1 | |a Longaker, M T |e verfasserin |4 aut | |
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