Protein loaded biodegradable microspheres based on PLGA-protein bioconjugates
Biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) was chemically conjugated to lysozyme, a model protein drug, by coupling a terminal carboxylic acid in PLGA with primary amine groups present in lysozyme. The conjugation was carried out in dimethylsulphoxide (DMSO) by using carbodiimide as a coupling agent. The PLGA-lysozyme conjugate, dissolved in a co-solvent system of DMSO and methylene chloride, was directly formulated into microspheres by an oil-in-water (O/W) single emulsion solvent evaporation technique. Morphological characteristics of the resultant microspheres, loading efficiencies, and protein release behaviours with protein instability problems were investigated in comparison with those of the microspheres prepared by water-in-oil-water (W/O/W) double emulsion and O/W single emulsion techniques which employed PLGA with unconjugated lysozyme for the formulation.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1999 |
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| Erschienen: |
1999 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
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| Enthalten in: |
Journal of microencapsulation - 16(1999), 5 vom: 21. Sept., Seite 625-37 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
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| Anmerkungen: |
Date Completed 29.12.1999 Date Revised 03.11.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM104307137 |
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| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
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| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Nam, Y S |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Protein loaded biodegradable microspheres based on PLGA-protein bioconjugates |
| 264 | 1 | |c 1999 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 29.12.1999 | ||
| 500 | |a Date Revised 03.11.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) was chemically conjugated to lysozyme, a model protein drug, by coupling a terminal carboxylic acid in PLGA with primary amine groups present in lysozyme. The conjugation was carried out in dimethylsulphoxide (DMSO) by using carbodiimide as a coupling agent. The PLGA-lysozyme conjugate, dissolved in a co-solvent system of DMSO and methylene chloride, was directly formulated into microspheres by an oil-in-water (O/W) single emulsion solvent evaporation technique. Morphological characteristics of the resultant microspheres, loading efficiencies, and protein release behaviours with protein instability problems were investigated in comparison with those of the microspheres prepared by water-in-oil-water (W/O/W) double emulsion and O/W single emulsion techniques which employed PLGA with unconjugated lysozyme for the formulation | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Biocompatible Materials |2 NLM | |
| 650 | 7 | |a Delayed-Action Preparations |2 NLM | |
| 650 | 7 | |a Polymers |2 NLM | |
| 650 | 7 | |a Solvents |2 NLM | |
| 650 | 7 | |a Polylactic Acid-Polyglycolic Acid Copolymer |2 NLM | |
| 650 | 7 | |a 1SIA8062RS |2 NLM | |
| 650 | 7 | |a Polyglycolic Acid |2 NLM | |
| 650 | 7 | |a 26009-03-0 |2 NLM | |
| 650 | 7 | |a Lactic Acid |2 NLM | |
| 650 | 7 | |a 33X04XA5AT |2 NLM | |
| 650 | 7 | |a Sodium Dodecyl Sulfate |2 NLM | |
| 650 | 7 | |a 368GB5141J |2 NLM | |
| 650 | 7 | |a Muramidase |2 NLM | |
| 650 | 7 | |a EC 3.2.1.17 |2 NLM | |
| 650 | 7 | |a Dimethyl Sulfoxide |2 NLM | |
| 650 | 7 | |a YOW8V9698H |2 NLM | |
| 700 | 1 | |a Park, T G |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of microencapsulation |d 1989 |g 16(1999), 5 vom: 21. Sept., Seite 625-37 |w (DE-627)NLM013098217 |x 1464-5246 |7 nnns |
| 773 | 1 | 8 | |g volume:16 |g year:1999 |g number:5 |g day:21 |g month:09 |g pages:625-37 |
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| 951 | |a AR | ||
| 952 | |d 16 |j 1999 |e 5 |b 21 |c 09 |h 625-37 | ||