Molecular cloning, expression and exon/intron organization of the bovine beta-galactoside alpha2,6-sialyltransferase gene
In this study, we report the first isolation and characterization of a bovine sialyltransferase gene. Bovine cDNAs prepared from different tissues contain an open-reading frame encoding a 405 amino acid sequence showing 83%, 75%, and 60% identity with human, murine, and chicken ST6Gal I (beta-galactoside alpha2,6-sialyltransferase) sequences, respectively. When transfected into COS-7 cells, a recombinant enzyme was obtained which catalyzed the in vitro alpha2, 6-sialylation of LacNAc (NeuAcalpha2-6Galbeta1-4GlcNAc) and LacdiNAc (NeuAcalpha2-6GalNAcbeta1-4GlcNAc) acceptor substrates. The K (m) values were 2.8 and 6.9 mM, respectively. Different relative efficiencies (Vmax/Km) for the two precursors (36 for LacNAc and 4.3 for LacdiNAc) were observed. Bovine ST6Gal I gene consists of four 5'-untranslated exons E(-2) to E(1), and five coding exons from E(2) to E(6). This later carries a 3'-untranslated region of 2. 7 kb. Gene sequence spans at least 80 kb of genomic DNA. Two processed pseudogenes have been identified. They are 94.3 and 95.6% similar to the bovine cDNA, respectively. Three families of mRNA isoforms were isolated. They differed by their 5'-untranslated regions and could be generated by three tissue-specific promoters. Family 1 is made up of exons E(-2) and E(1) to E(6), family 2 of exons E(-1) to E(6), and family 3 of exons E(1) to E(6). Tissular distribution of transcript families appears noticeably different than those described in human and rat.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1999 |
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| Erschienen: |
1999 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
|---|---|
| Enthalten in: |
Glycobiology - 9(1999), 9 vom: 26. Sept., Seite 851-63 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Mercier, D [VerfasserIn] |
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| Anmerkungen: |
Date Completed 04.11.1999 Date Revised 07.12.2022 published: Print GENBANK: AJ006830, AJ006831, AJ006832, AJ006833 Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM103921125 |
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| 003 | DE-627 | ||
| 005 | 20231222131421.0 | ||
| 007 | tu | ||
| 008 | 231222s1999 xx ||||| 00| ||eng c | ||
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| 035 | |a (DE-627)NLM103921125 | ||
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| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Mercier, D |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Molecular cloning, expression and exon/intron organization of the bovine beta-galactoside alpha2,6-sialyltransferase gene |
| 264 | 1 | |c 1999 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 04.11.1999 | ||
| 500 | |a Date Revised 07.12.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a GENBANK: AJ006830, AJ006831, AJ006832, AJ006833 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a In this study, we report the first isolation and characterization of a bovine sialyltransferase gene. Bovine cDNAs prepared from different tissues contain an open-reading frame encoding a 405 amino acid sequence showing 83%, 75%, and 60% identity with human, murine, and chicken ST6Gal I (beta-galactoside alpha2,6-sialyltransferase) sequences, respectively. When transfected into COS-7 cells, a recombinant enzyme was obtained which catalyzed the in vitro alpha2, 6-sialylation of LacNAc (NeuAcalpha2-6Galbeta1-4GlcNAc) and LacdiNAc (NeuAcalpha2-6GalNAcbeta1-4GlcNAc) acceptor substrates. The K (m) values were 2.8 and 6.9 mM, respectively. Different relative efficiencies (Vmax/Km) for the two precursors (36 for LacNAc and 4.3 for LacdiNAc) were observed. Bovine ST6Gal I gene consists of four 5'-untranslated exons E(-2) to E(1), and five coding exons from E(2) to E(6). This later carries a 3'-untranslated region of 2. 7 kb. Gene sequence spans at least 80 kb of genomic DNA. Two processed pseudogenes have been identified. They are 94.3 and 95.6% similar to the bovine cDNA, respectively. Three families of mRNA isoforms were isolated. They differed by their 5'-untranslated regions and could be generated by three tissue-specific promoters. Family 1 is made up of exons E(-2) and E(1) to E(6), family 2 of exons E(-1) to E(6), and family 3 of exons E(1) to E(6). Tissular distribution of transcript families appears noticeably different than those described in human and rat | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a 5' Untranslated Regions |2 NLM | |
| 650 | 7 | |a Antigens, CD |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a Recombinant Proteins |2 NLM | |
| 650 | 7 | |a Retroelements |2 NLM | |
| 650 | 7 | |a Sialyltransferases |2 NLM | |
| 650 | 7 | |a EC 2.4.99.- |2 NLM | |
| 650 | 7 | |a beta-D-Galactoside alpha 2-6-Sialyltransferase |2 NLM | |
| 650 | 7 | |a EC 2.4.99.1 |2 NLM | |
| 700 | 1 | |a Wierinckx, A |e verfasserin |4 aut | |
| 700 | 1 | |a Oulmouden, A |e verfasserin |4 aut | |
| 700 | 1 | |a Gallet, P F |e verfasserin |4 aut | |
| 700 | 1 | |a Palcic, M M |e verfasserin |4 aut | |
| 700 | 1 | |a Harduin-Lepers, A |e verfasserin |4 aut | |
| 700 | 1 | |a Delannoy, P |e verfasserin |4 aut | |
| 700 | 1 | |a Petit, J M |e verfasserin |4 aut | |
| 700 | 1 | |a Levéziel, H |e verfasserin |4 aut | |
| 700 | 1 | |a Julien, R |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Glycobiology |d 1990 |g 9(1999), 9 vom: 26. Sept., Seite 851-63 |w (DE-627)NLM012612502 |x 1460-2423 |7 nnns |
| 773 | 1 | 8 | |g volume:9 |g year:1999 |g number:9 |g day:26 |g month:09 |g pages:851-63 |
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