Shigella-induced apoptosis is dependent on caspase-1 which binds to IpaB
We report here that the Shigella invasion plasmid antigen (Ipa)B, which is sufficient to induce apoptosis in macrophages, binds to caspase (Casp)-1, but not to Casp-2 or Casp-3. Casp-1 is activated and its specific substrate interleukin-1beta is cleaved shortly after Shigella infection. Macrophages isolated from Casp-1 knock-out mice are not susceptible to Shigella-induced apoptosis, although they respond normally to other apoptotic stimuli. Shigella kills macrophages from casp-3, casp-11, and p53 knock-out mice as well as macrophages overexpressing Bcl-2. We propose that Shigella induces apoptosis by directly activating Casp-1 through IpaB, bypassing signal transduction events and caspases upstream of Casp-1. Taken together these data indicate that Shigella-induced apoptosis is distinct from other forms of apoptosis and seems uniquely dependent on Casp-1.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
1998 |
|---|---|
| Erschienen: |
1998 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:273 |
|---|---|
| Enthalten in: |
The Journal of biological chemistry - 273(1998), 49 vom: 04. Dez., Seite 32895-900 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Hilbi, H [VerfasserIn] |
|---|
| Anmerkungen: |
Date Completed 08.01.1999 Date Revised 09.02.2021 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM09767799X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM09767799X | ||
| 003 | DE-627 | ||
| 005 | 20231222110448.0 | ||
| 007 | tu | ||
| 008 | 231222s1998 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0326.xml |
| 035 | |a (DE-627)NLM09767799X | ||
| 035 | |a (NLM)9830039 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Hilbi, H |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Shigella-induced apoptosis is dependent on caspase-1 which binds to IpaB |
| 264 | 1 | |c 1998 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 08.01.1999 | ||
| 500 | |a Date Revised 09.02.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a We report here that the Shigella invasion plasmid antigen (Ipa)B, which is sufficient to induce apoptosis in macrophages, binds to caspase (Casp)-1, but not to Casp-2 or Casp-3. Casp-1 is activated and its specific substrate interleukin-1beta is cleaved shortly after Shigella infection. Macrophages isolated from Casp-1 knock-out mice are not susceptible to Shigella-induced apoptosis, although they respond normally to other apoptotic stimuli. Shigella kills macrophages from casp-3, casp-11, and p53 knock-out mice as well as macrophages overexpressing Bcl-2. We propose that Shigella induces apoptosis by directly activating Casp-1 through IpaB, bypassing signal transduction events and caspases upstream of Casp-1. Taken together these data indicate that Shigella-induced apoptosis is distinct from other forms of apoptosis and seems uniquely dependent on Casp-1 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a Proto-Oncogene Proteins c-bcl-2 |2 NLM | |
| 650 | 7 | |a Tumor Suppressor Protein p53 |2 NLM | |
| 650 | 7 | |a ipaB protein, Shigella |2 NLM | |
| 650 | 7 | |a 127384-62-7 |2 NLM | |
| 650 | 7 | |a Caspase 1 |2 NLM | |
| 650 | 7 | |a EC 3.4.22.36 |2 NLM | |
| 700 | 1 | |a Moss, J E |e verfasserin |4 aut | |
| 700 | 1 | |a Hersh, D |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Y |e verfasserin |4 aut | |
| 700 | 1 | |a Arondel, J |e verfasserin |4 aut | |
| 700 | 1 | |a Banerjee, S |e verfasserin |4 aut | |
| 700 | 1 | |a Flavell, R A |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, J |e verfasserin |4 aut | |
| 700 | 1 | |a Sansonetti, P J |e verfasserin |4 aut | |
| 700 | 1 | |a Zychlinsky, A |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The Journal of biological chemistry |d 1945 |g 273(1998), 49 vom: 04. Dez., Seite 32895-900 |w (DE-627)NLM000004995 |x 1083-351X |7 nnns |
| 773 | 1 | 8 | |g volume:273 |g year:1998 |g number:49 |g day:04 |g month:12 |g pages:32895-900 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 273 |j 1998 |e 49 |b 04 |c 12 |h 32895-900 | ||