Physico-chemical characterization of a double long-chain cationic amphiphile (Vectamidine) by microelectrophoresis
We recently synthesized a novel cationic amphiphile (N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine or Vectamidine (previously described as diC14-amidine)) that associates with DNA and RNA and facilitates their entry and expression into eukaryotic cells. Among several parameters that have been shown to influence the transfection process, the surface charge density plays a key role. Quantitative information about that charge density associated to the cationic amphiphiles organized in liposomal structure is not yet available. We provide here evidence by titration and microelectrophoresis measurements that an evaluation of the intrinsic acidity constants, the surface pH and the counterion binding constants allows to determine the charge density at physiological pH of Vectamidine liposomes. The knowledge of this superficial charge is a prerequisite to a molecular understanding of the DNA-cationic amphiphile complex formation. The method described could be extended to any kind of cationic amphiphile.
Medienart: |
Artikel |
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Erscheinungsjahr: |
1998 |
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Erschienen: |
1998 |
Enthalten in: |
Zur Gesamtaufnahme - volume:1372 |
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Enthalten in: |
Biochimica et biophysica acta - 1372(1998), 2 vom: 17. Juli, Seite 339-46 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Pector, V [VerfasserIn] |
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Themen: |
63231-63-0 |
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Anmerkungen: |
Date Completed 04.09.1998 Date Revised 10.06.2019 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM096149787 |
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100 | 1 | |a Pector, V |e verfasserin |4 aut | |
245 | 1 | 0 | |a Physico-chemical characterization of a double long-chain cationic amphiphile (Vectamidine) by microelectrophoresis |
264 | 1 | |c 1998 | |
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500 | |a Date Completed 04.09.1998 | ||
500 | |a Date Revised 10.06.2019 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a We recently synthesized a novel cationic amphiphile (N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine or Vectamidine (previously described as diC14-amidine)) that associates with DNA and RNA and facilitates their entry and expression into eukaryotic cells. Among several parameters that have been shown to influence the transfection process, the surface charge density plays a key role. Quantitative information about that charge density associated to the cationic amphiphiles organized in liposomal structure is not yet available. We provide here evidence by titration and microelectrophoresis measurements that an evaluation of the intrinsic acidity constants, the surface pH and the counterion binding constants allows to determine the charge density at physiological pH of Vectamidine liposomes. The knowledge of this superficial charge is a prerequisite to a molecular understanding of the DNA-cationic amphiphile complex formation. The method described could be extended to any kind of cationic amphiphile | ||
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700 | 1 | |a Caspers, J |e verfasserin |4 aut | |
700 | 1 | |a Banerjee, S |e verfasserin |4 aut | |
700 | 1 | |a Deriemaeker, L |e verfasserin |4 aut | |
700 | 1 | |a Fuks, R |e verfasserin |4 aut | |
700 | 1 | |a El Ouahabi, A |e verfasserin |4 aut | |
700 | 1 | |a Vandenbranden, M |e verfasserin |4 aut | |
700 | 1 | |a Finsy, R |e verfasserin |4 aut | |
700 | 1 | |a Ruysschaert, J M |e verfasserin |4 aut | |
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