Molecular cloning of a hemopexin-like cDNA from rainbow trout liver
Hemopexin is an abundant heme-binding serum protein synthesized in the liver and for which the sequence is determined only for mammals. Little is known about fish heme-binding proteins. We have cloned a rainbow trout complementary DNA that encodes a 445 amino acid polypeptide exhibiting an overall 30% homology with human hemopexin and 69% with the goldfish warm temperature acclimation-related 65-kDa protein. Structural homology, deduced from hydrophobic cluster analysis (HCA), was strong between the trout and human proteins since global HCA scores of 76% were obtained when the N- or C-terminal halves of the two proteins were compared. Moreover, several characteristics of hemopexin were found in the trout protein; finally, the trout hemopexin-like messenger RNA was specifically expressed in the liver. We conclude that the trout protein is a good candidate for a true fish hemopexin.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1996 |
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| Erschienen: |
1996 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:6 |
|---|---|
| Enthalten in: |
DNA sequence : the journal of DNA sequencing and mapping - 6(1996), 6 vom: 24., Seite 311-8 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Miot, S [VerfasserIn] |
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| Themen: |
9013-71-2 |
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| Anmerkungen: |
Date Completed 07.07.1997 Date Revised 24.10.2019 published: Print GENBANK: Z68112 Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM089544056 |
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| 100 | 1 | |a Miot, S |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Molecular cloning of a hemopexin-like cDNA from rainbow trout liver |
| 264 | 1 | |c 1996 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
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| 500 | |a Date Completed 07.07.1997 | ||
| 500 | |a Date Revised 24.10.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a GENBANK: Z68112 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Hemopexin is an abundant heme-binding serum protein synthesized in the liver and for which the sequence is determined only for mammals. Little is known about fish heme-binding proteins. We have cloned a rainbow trout complementary DNA that encodes a 445 amino acid polypeptide exhibiting an overall 30% homology with human hemopexin and 69% with the goldfish warm temperature acclimation-related 65-kDa protein. Structural homology, deduced from hydrophobic cluster analysis (HCA), was strong between the trout and human proteins since global HCA scores of 76% were obtained when the N- or C-terminal halves of the two proteins were compared. Moreover, several characteristics of hemopexin were found in the trout protein; finally, the trout hemopexin-like messenger RNA was specifically expressed in the liver. We conclude that the trout protein is a good candidate for a true fish hemopexin | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a DNA Probes |2 NLM | |
| 650 | 7 | |a DNA, Complementary |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a Hemopexin |2 NLM | |
| 650 | 7 | |a 9013-71-2 |2 NLM | |
| 700 | 1 | |a Duval, J |e verfasserin |4 aut | |
| 700 | 1 | |a Le Goff, P |e verfasserin |4 aut | |
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