Morphological correlates of altered neuronal activity in organotypic cerebellar cultures chronically exposed to anti-GABA agents
Organotypic cerebellar cultures derived from newborn mice were chronically exposed to medium containing picrotoxin or bicuculline from explanation until they were recorded from extracellularly or fixed for morphological studies. Cultures exposed to anti-GABA agents for 13-18 days in vitro had decreased spontaneous cortical discharge rates when compared with sister control cultures and prolonged inhibitory responses to cortical stimulation. Electron microscopic examination of exposed cultures after 14-16 days in vitro revealed a hyperinnervation of Purkinje cell somata by inhibitory terminals predominantly of basket cell origin. The sprouted terminals penetrated otherwise intact Purkinje cell astrocytic sheaths. These changes represent a departure from the usual developmental pattern, a departure induced by exposure to anti-GABA agents that increased neuronal activity early in the development of the cerebellar circuitry in vitro. The precise signals that initiated the changes are unknown, but the altered development is most likely in response to increased Purkinje cell activity.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1994 |
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| Erschienen: |
1994 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:77 |
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| Enthalten in: |
Brain research. Developmental brain research - 77(1994), 1 vom: 14. Jan., Seite 123-32 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Seil, F J [VerfasserIn] |
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| Anmerkungen: |
Date Completed 19.04.1994 Date Revised 30.08.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM07882947X |
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| 100 | 1 | |a Seil, F J |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Morphological correlates of altered neuronal activity in organotypic cerebellar cultures chronically exposed to anti-GABA agents |
| 264 | 1 | |c 1994 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 19.04.1994 | ||
| 500 | |a Date Revised 30.08.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Organotypic cerebellar cultures derived from newborn mice were chronically exposed to medium containing picrotoxin or bicuculline from explanation until they were recorded from extracellularly or fixed for morphological studies. Cultures exposed to anti-GABA agents for 13-18 days in vitro had decreased spontaneous cortical discharge rates when compared with sister control cultures and prolonged inhibitory responses to cortical stimulation. Electron microscopic examination of exposed cultures after 14-16 days in vitro revealed a hyperinnervation of Purkinje cell somata by inhibitory terminals predominantly of basket cell origin. The sprouted terminals penetrated otherwise intact Purkinje cell astrocytic sheaths. These changes represent a departure from the usual developmental pattern, a departure induced by exposure to anti-GABA agents that increased neuronal activity early in the development of the cerebellar circuitry in vitro. The precise signals that initiated the changes are unknown, but the altered development is most likely in response to increased Purkinje cell activity | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
| 650 | 7 | |a GABA Antagonists |2 NLM | |
| 650 | 7 | |a Quinoxalines |2 NLM | |
| 650 | 7 | |a Picrotoxin |2 NLM | |
| 650 | 7 | |a 124-87-8 |2 NLM | |
| 650 | 7 | |a 6-Cyano-7-nitroquinoxaline-2,3-dione |2 NLM | |
| 650 | 7 | |a 6OTE87SCCW |2 NLM | |
| 650 | 7 | |a Bicuculline |2 NLM | |
| 650 | 7 | |a Y37615DVKC |2 NLM | |
| 700 | 1 | |a Drake-Baumann, R |e verfasserin |4 aut | |
| 700 | 1 | |a Leiman, A L |e verfasserin |4 aut | |
| 700 | 1 | |a Herndon, R M |e verfasserin |4 aut | |
| 700 | 1 | |a Tiekotter, K L |e verfasserin |4 aut | |
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