Molecular construction of Clostridium botulinum type C progenitor toxin and its gene organization
The 16S progenitor toxin of Clostridium botulinum type C is made up by conjugation of a neurotoxin with nontoxic components designated as nontoxic-nonHA and hemagglutinin (HA). The HA was found to be composed of subcomponents having 53, 33, 22-23, and 17 kDa molecular masses. Since we previously determined the whole nucleotide sequences of the genes for neurotoxin, nontoxic-nonHA, and HA-33, the cloning and nucleotide sequencing of the genes for the remaining HA subcomponents were performed. Two open reading frames coding for 16.7 kDa (HA-17) and 70.6 kDa proteins were identified. The N-terminal amino acid sequences of HA-53 and HA-22-23 indicated that the 70.6 kDa protein is split into 53 and the 22-23 kDa proteins after translation and that the 22-23 kDa protein consists of at least four proteins showing slightly different molecular weights.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
1994 |
|---|---|
| Erschienen: |
1994 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:205 |
|---|---|
| Enthalten in: |
Biochemical and biophysical research communications - 205(1994), 2 vom: 15. Dez., Seite 1291-8 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Fujinaga, Y [VerfasserIn] |
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| Themen: |
Botulinum Toxins |
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| Anmerkungen: |
Date Completed 25.01.1995 Date Revised 18.11.2010 published: Print GENBANK: D38562 Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM077783484 |
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| 041 | |a eng | ||
| 100 | 1 | |a Fujinaga, Y |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Molecular construction of Clostridium botulinum type C progenitor toxin and its gene organization |
| 264 | 1 | |c 1994 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 25.01.1995 | ||
| 500 | |a Date Revised 18.11.2010 | ||
| 500 | |a published: Print | ||
| 500 | |a GENBANK: D38562 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The 16S progenitor toxin of Clostridium botulinum type C is made up by conjugation of a neurotoxin with nontoxic components designated as nontoxic-nonHA and hemagglutinin (HA). The HA was found to be composed of subcomponents having 53, 33, 22-23, and 17 kDa molecular masses. Since we previously determined the whole nucleotide sequences of the genes for neurotoxin, nontoxic-nonHA, and HA-33, the cloning and nucleotide sequencing of the genes for the remaining HA subcomponents were performed. Two open reading frames coding for 16.7 kDa (HA-17) and 70.6 kDa proteins were identified. The N-terminal amino acid sequences of HA-53 and HA-22-23 indicated that the 70.6 kDa protein is split into 53 and the 22-23 kDa proteins after translation and that the 22-23 kDa protein consists of at least four proteins showing slightly different molecular weights | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a DNA, Bacterial |2 NLM | |
| 650 | 7 | |a Hemagglutinins |2 NLM | |
| 650 | 7 | |a Macromolecular Substances |2 NLM | |
| 650 | 7 | |a Botulinum Toxins |2 NLM | |
| 650 | 7 | |a EC 3.4.24.69 |2 NLM | |
| 700 | 1 | |a Inoue, K |e verfasserin |4 aut | |
| 700 | 1 | |a Shimazaki, S |e verfasserin |4 aut | |
| 700 | 1 | |a Tomochika, K |e verfasserin |4 aut | |
| 700 | 1 | |a Tsuzuki, K |e verfasserin |4 aut | |
| 700 | 1 | |a Fujii, N |e verfasserin |4 aut | |
| 700 | 1 | |a Watanabe, T |e verfasserin |4 aut | |
| 700 | 1 | |a Ohyama, T |e verfasserin |4 aut | |
| 700 | 1 | |a Takeshi, K |e verfasserin |4 aut | |
| 700 | 1 | |a Inoue, K |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biochemical and biophysical research communications |d 1960 |g 205(1994), 2 vom: 15. Dez., Seite 1291-8 |w (DE-627)NLM000000035 |x 1090-2104 |7 nnns |
| 773 | 1 | 8 | |g volume:205 |g year:1994 |g number:2 |g day:15 |g month:12 |g pages:1291-8 |
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| 952 | |d 205 |j 1994 |e 2 |b 15 |c 12 |h 1291-8 | ||