Biological evaluation of an ionomeric bone cement by osteoblast cell culture methods
Periosteal derived bovine osteoblast-like cells migrated in culture onto an ionomeric cement. Cell cultures were maintained for 4 weeks and used to study the in vitro behaviour of cells on the ionomeric bone cement (IC). The cells produced bone matrix proteins (osteocalcin, bone sialoprotein II) and were osteoblast-like. The osteoblast-like cells colonized the substrate in monolayers and produced an extracellular matrix as seen by light and scanning electron microscopy. Morphological comparison between cells growing on the ionomeric bone cement and cortical bone revealed no significant difference in phenotypic expression. Staining for aluminium in osteoblasts growing on the IC showed an uptake and storage of aluminium in the cells. Energy dispersive X-ray microanalysis revealed high concentrations of aluminium and silicon in the periosteal tissue. Despite the known toxic effect of aluminium in vivo and in vitro on osteoblasts, no signs of toxicity were apparent on light and scanning electron microscopy analysis.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1993 |
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| Erschienen: |
1993 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Biomaterials - 14(1993), 12 vom: 14. Okt., Seite 917-24 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Meyer, U [VerfasserIn] |
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| Themen: |
Aluminum Oxide |
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| Anmerkungen: |
Date Completed 01.02.1994 Date Revised 15.08.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM074820095 |
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| 100 | 1 | |a Meyer, U |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Biological evaluation of an ionomeric bone cement by osteoblast cell culture methods |
| 264 | 1 | |c 1993 | |
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| 500 | |a Date Completed 01.02.1994 | ||
| 500 | |a Date Revised 15.08.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Periosteal derived bovine osteoblast-like cells migrated in culture onto an ionomeric cement. Cell cultures were maintained for 4 weeks and used to study the in vitro behaviour of cells on the ionomeric bone cement (IC). The cells produced bone matrix proteins (osteocalcin, bone sialoprotein II) and were osteoblast-like. The osteoblast-like cells colonized the substrate in monolayers and produced an extracellular matrix as seen by light and scanning electron microscopy. Morphological comparison between cells growing on the ionomeric bone cement and cortical bone revealed no significant difference in phenotypic expression. Staining for aluminium in osteoblasts growing on the IC showed an uptake and storage of aluminium in the cells. Energy dispersive X-ray microanalysis revealed high concentrations of aluminium and silicon in the periosteal tissue. Despite the known toxic effect of aluminium in vivo and in vitro on osteoblasts, no signs of toxicity were apparent on light and scanning electron microscopy analysis | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Biocompatible Materials |2 NLM | |
| 650 | 7 | |a Bone Cements |2 NLM | |
| 650 | 7 | |a Aluminum Oxide |2 NLM | |
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| 650 | 7 | |a Silicon |2 NLM | |
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| 700 | 1 | |a Szulczewski, D H |e verfasserin |4 aut | |
| 700 | 1 | |a Barckhaus, R H |e verfasserin |4 aut | |
| 700 | 1 | |a Atkinson, M |e verfasserin |4 aut | |
| 700 | 1 | |a Jones, D B |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biomaterials |d 1989 |g 14(1993), 12 vom: 14. Okt., Seite 917-24 |w (DE-627)NLM012732516 |x 1878-5905 |7 nnns |
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