The presence of glycosyltransferases on chromatin acceptors in monkey liver nuclear membranes (author's transl)
Nuclei were prepared from monkey hepatocytes by centrifugation of the homogenate on a cushion of 2.3 M sucrose, during 45 min at 100000 X g. The yield was 2.2 x 10(7) nuclei per g of liver, and 70% of te homogenate DNA was recovered in these nuclei. An electron microscopic study as well as a biochemical analysis of marker enzymes showed that the nuclei are not contaminated by other subcellular fractions, especially endoplasmic reticulum. A mannosyltransferase and an N-acetylglucosaminyltransferase, working on endogenous glycoproteic acceptors, are present in the nuclei for 1.4 and 6.5% of the homogenate activities, respectively. The nuclei are hydrolysed by DNAse I. The suspension, adjusted in 1.9 M sucrose, was centrifuged for 2 h at 100000 X g, under buffer layer. Purified nuclear membranes were collected at the interface. These membranes did not contain any more endoplasmic reticulum enzyme activities, but the mannosyl and N-acetylglucosaminyltransferase activities were still present. They essentially work on an exogenous chromatin acceptor, prepared by lysis of the nuclei. The eventual role of these glycosyltransferases in the glycosylation of non-histone proteins is discussed.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
1980 |
|---|---|
| Erschienen: |
1980 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:603 |
|---|---|
| Enthalten in: |
Biochimica et biophysica acta - 603(1980), 2 vom: 12. Dez., Seite 245-54 |
| Sprache: |
Französisch |
|---|
| Weiterer Titel: |
Présence de glycosyltransferases à accepteurs chromatiniens dans les membranes nucléaires d'hépatocytes de singe |
|---|
| Beteiligte Personen: |
Berthillier, G [VerfasserIn] |
|---|
| Anmerkungen: |
Date Completed 24.04.1981 Date Revised 09.06.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
|
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| PPN (Katalog-ID): |
NLM064276090 |
|---|
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| 100 | 1 | |a Berthillier, G |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The presence of glycosyltransferases on chromatin acceptors in monkey liver nuclear membranes (author's transl) |
| 246 | 3 | 3 | |a Présence de glycosyltransferases à accepteurs chromatiniens dans les membranes nucléaires d'hépatocytes de singe |
| 264 | 1 | |c 1980 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 24.04.1981 | ||
| 500 | |a Date Revised 09.06.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Nuclei were prepared from monkey hepatocytes by centrifugation of the homogenate on a cushion of 2.3 M sucrose, during 45 min at 100000 X g. The yield was 2.2 x 10(7) nuclei per g of liver, and 70% of te homogenate DNA was recovered in these nuclei. An electron microscopic study as well as a biochemical analysis of marker enzymes showed that the nuclei are not contaminated by other subcellular fractions, especially endoplasmic reticulum. A mannosyltransferase and an N-acetylglucosaminyltransferase, working on endogenous glycoproteic acceptors, are present in the nuclei for 1.4 and 6.5% of the homogenate activities, respectively. The nuclei are hydrolysed by DNAse I. The suspension, adjusted in 1.9 M sucrose, was centrifuged for 2 h at 100000 X g, under buffer layer. Purified nuclear membranes were collected at the interface. These membranes did not contain any more endoplasmic reticulum enzyme activities, but the mannosyl and N-acetylglucosaminyltransferase activities were still present. They essentially work on an exogenous chromatin acceptor, prepared by lysis of the nuclei. The eventual role of these glycosyltransferases in the glycosylation of non-histone proteins is discussed | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Chromatin |2 NLM | |
| 650 | 7 | |a Glucosyltransferases |2 NLM | |
| 650 | 7 | |a EC 2.4.1.- |2 NLM | |
| 650 | 7 | |a Hexosyltransferases |2 NLM | |
| 650 | 7 | |a EC 2.4.1.- |2 NLM | |
| 650 | 7 | |a Mannosyltransferases |2 NLM | |
| 650 | 7 | |a EC 2.4.1.- |2 NLM | |
| 650 | 7 | |a N-Acetylglucosaminyltransferases |2 NLM | |
| 650 | 7 | |a EC 2.4.1.- |2 NLM | |
| 650 | 7 | |a N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase |2 NLM | |
| 650 | 7 | |a EC 2.4.1.150 |2 NLM | |
| 650 | 7 | |a Acetylglucosamine |2 NLM | |
| 650 | 7 | |a V956696549 |2 NLM | |
| 700 | 1 | |a Benedetto, J P |e verfasserin |4 aut | |
| 700 | 1 | |a Got, R |e verfasserin |4 aut | |
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