Titration of the binding sites for the oligomycin-sensitivity conferring protein in beef heart submitochondrial particles

The binding parameters of the oligomycin-sensitivity conferring protein (OSCP) in inside-out particles from beef heart mitochondria have been tested by means of two assays, the oligomycin-sensitive ATP-Pi exchange, and the oligomycin-sensitive ATP hydrolysis. The total number of OSCP binding sites in A particles was equal to 220 pmol/mg particle protein. Each mole of ATPase active site was able to bind 1.1 +/- 0.5 mol OSCP with Kd 1.7 nM.

Medienart:

Artikel

Erscheinungsjahr:

1983

Erschienen:

1983

Enthalten in:

Zur Gesamtaufnahme - volume:156

Enthalten in:

FEBS letters - 156(1983), 1 vom: 30. Mai, Seite 99-102

Sprache:

Englisch

Beteiligte Personen:

Dupuis, A [VerfasserIn]
Satre, M [VerfasserIn]
Vignais, P V [VerfasserIn]

Themen:

8L70Q75FXE
Adenosine Triphosphatases
Adenosine Triphosphate
Carrier Proteins
EC 3.6.1.-
EC 3.6.3.-
EC 3.6.3.14
EC 7.1.2.2
Journal Article
Membrane Proteins
Mitochondrial Proton-Translocating ATPases
Oligomycin sensitivity-conferring protein
Oligomycins
Phosphates
Proton-Translocating ATPases

Anmerkungen:

Date Completed 15.07.1983

Date Revised 10.12.2019

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM061668699