Structure and expression of a gene encoding the precursor of subtilin, a small protein antibiotic
A gene has been cloned from Bacillus subtilis ATCC 6633 that encodes a 56-residue peptide precursor for the 32-residue peptide antibiotic, subtilin. The precursor contains serines, threonines, and cysteines at positions that permit them to undergo a series of dehydration and cross-linking steps to give the mature antibiotic peptide which contains the unusual amino acids lanthionine, beta-methyllanthionine, D-alanine, dehydroalanine, and dehydrobutyrine. The precursor peptide contains a leader region that has an unusual hydropathic character for an exported protein. The subtilin gene is expressed from a monocistronic transcriptional unit as identified by S1 mapping of the gene transcript. The (-35) region of the promoter is not typical of Bacillus subtilis vegetatively-expressed genes. The subtilin transcript was present in very low amounts during exponential growth, but in large amounts during stationary phase; at which time mature subtilin peptide and antibiotic activity were also observed. The subtilin transcript contains a cleavage-sensitive region that overlaps the ribosome binding site. The primary transcript has an unusually long half-life of about 45 min. These observations confirm that subtilin is derived from a small protein that is synthesized on ribosomes.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1988 |
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| Erschienen: |
1988 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:263 |
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| Enthalten in: |
The Journal of biological chemistry - 263(1988), 19 vom: 05. Juli, Seite 9508-14 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Banerjee, S [VerfasserIn] |
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| Anmerkungen: |
Date Completed 27.07.1988 Date Revised 10.02.2021 published: Print GENBANK: J03767 Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM028188144 |
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| 041 | |a eng | ||
| 100 | 1 | |a Banerjee, S |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Structure and expression of a gene encoding the precursor of subtilin, a small protein antibiotic |
| 264 | 1 | |c 1988 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 27.07.1988 | ||
| 500 | |a Date Revised 10.02.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a GENBANK: J03767 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A gene has been cloned from Bacillus subtilis ATCC 6633 that encodes a 56-residue peptide precursor for the 32-residue peptide antibiotic, subtilin. The precursor contains serines, threonines, and cysteines at positions that permit them to undergo a series of dehydration and cross-linking steps to give the mature antibiotic peptide which contains the unusual amino acids lanthionine, beta-methyllanthionine, D-alanine, dehydroalanine, and dehydrobutyrine. The precursor peptide contains a leader region that has an unusual hydropathic character for an exported protein. The subtilin gene is expressed from a monocistronic transcriptional unit as identified by S1 mapping of the gene transcript. The (-35) region of the promoter is not typical of Bacillus subtilis vegetatively-expressed genes. The subtilin transcript was present in very low amounts during exponential growth, but in large amounts during stationary phase; at which time mature subtilin peptide and antibiotic activity were also observed. The subtilin transcript contains a cleavage-sensitive region that overlaps the ribosome binding site. The primary transcript has an unusually long half-life of about 45 min. These observations confirm that subtilin is derived from a small protein that is synthesized on ribosomes | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
| 650 | 7 | |a Anti-Bacterial Agents |2 NLM | |
| 650 | 7 | |a Antimicrobial Cationic Peptides |2 NLM | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a Bacteriocins |2 NLM | |
| 650 | 7 | |a Peptides |2 NLM | |
| 650 | 7 | |a Protein Precursors |2 NLM | |
| 650 | 7 | |a DNA Restriction Enzymes |2 NLM | |
| 650 | 7 | |a EC 3.1.21.- |2 NLM | |
| 650 | 7 | |a subtilin |2 NLM | |
| 650 | 7 | |a JCA69WH3TT |2 NLM | |
| 700 | 1 | |a Hansen, J N |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The Journal of biological chemistry |d 1945 |g 263(1988), 19 vom: 05. Juli, Seite 9508-14 |w (DE-627)NLM000004995 |x 1083-351X |7 nnns |
| 773 | 1 | 8 | |g volume:263 |g year:1988 |g number:19 |g day:05 |g month:07 |g pages:9508-14 |
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| 952 | |d 263 |j 1988 |e 19 |b 05 |c 07 |h 9508-14 | ||