Immunohistochemical analysis of brain macrophages in adrenoleukodystrophy
Using a panel of monoclonal antibodies directed against antigenic determinants present at the surface of mononuclear cells, we characterized the macrophage infiltration in frozen brain specimens from a patient with adrenoleukodystrophy by immunohistochemical staining. This study demonstrated that macrophages were the main infiltrating cells in the white matter and that there was a wide variability in their phenotype according to their location: they stained very strongly with Leu-M5, EBM11, Leu-M3, To5 and 2LPM 19 C in the perivascular cuffs, whereas they stained principally with Leu-M5 and EBM11 and to a lesser extent with To5 and 2LPM19c in the white matter. In addition to the expression of MHC class II and CD4 molecules, macrophages exhibited strong labelling with 7G7B6, a monoclonal antibody raised against IL2 receptors. These results attest that macrophages, which infiltrate the brain parenchyma have various phenotypes, and very likely different states of activation, and suggest that interleukin-2 plays an important role during this activation.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
1989 |
|---|---|
| Erschienen: |
1989 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
|---|---|
| Enthalten in: |
Neuropediatrics - 20(1989), 4 vom: 30. Nov., Seite 202-6 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Boutin, B [VerfasserIn] |
|---|
| Themen: |
Antibodies, Monoclonal |
|---|
| Anmerkungen: |
Date Completed 12.02.1990 Date Revised 19.11.2015 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM025894978 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM025894978 | ||
| 003 | DE-627 | ||
| 005 | 20231221100410.0 | ||
| 007 | tu | ||
| 008 | 231221s1989 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0087.xml |
| 035 | |a (DE-627)NLM025894978 | ||
| 035 | |a (NLM)2608166 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Boutin, B |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Immunohistochemical analysis of brain macrophages in adrenoleukodystrophy |
| 264 | 1 | |c 1989 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 12.02.1990 | ||
| 500 | |a Date Revised 19.11.2015 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Using a panel of monoclonal antibodies directed against antigenic determinants present at the surface of mononuclear cells, we characterized the macrophage infiltration in frozen brain specimens from a patient with adrenoleukodystrophy by immunohistochemical staining. This study demonstrated that macrophages were the main infiltrating cells in the white matter and that there was a wide variability in their phenotype according to their location: they stained very strongly with Leu-M5, EBM11, Leu-M3, To5 and 2LPM 19 C in the perivascular cuffs, whereas they stained principally with Leu-M5 and EBM11 and to a lesser extent with To5 and 2LPM19c in the white matter. In addition to the expression of MHC class II and CD4 molecules, macrophages exhibited strong labelling with 7G7B6, a monoclonal antibody raised against IL2 receptors. These results attest that macrophages, which infiltrate the brain parenchyma have various phenotypes, and very likely different states of activation, and suggest that interleukin-2 plays an important role during this activation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a Antigens, Differentiation |2 NLM | |
| 700 | 1 | |a Matsuguchi, L |e verfasserin |4 aut | |
| 700 | 1 | |a Lebon, P |e verfasserin |4 aut | |
| 700 | 1 | |a Ponsot, G |e verfasserin |4 aut | |
| 700 | 1 | |a Arthuis, C |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Neuropediatrics |d 1993 |g 20(1989), 4 vom: 30. Nov., Seite 202-6 |w (DE-627)NLM012996483 |x 1439-1899 |7 nnns |
| 773 | 1 | 8 | |g volume:20 |g year:1989 |g number:4 |g day:30 |g month:11 |g pages:202-6 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 20 |j 1989 |e 4 |b 30 |c 11 |h 202-6 | ||