Biosynthesis of the glycosyl phosphatidylinositol membrane anchor of the trypanosome variant surface glycoprotein. Origin of the non-acetylated glucosamine
Non-acetylated glucosamine is an unusual structural feature shared by all glycosyl phosphatidylinositol (GPI) lipids, including a variety of membrane anchors, the leishmanial lipophosphoglycan, and a mediator of insulin action. We proposed previously a pathway for biosynthesis of glycolipid A, the precursor of the GPI membrane anchor of the trypanosome variant surface glycoprotein (Masterson, W. J., Doering, T. L., Hart, G. W., and Englund, P. T. (1989) Cell 56, 793-800). In this paper we characterize in more detail the initial steps of GPI assembly. The first and committed step in the pathway is the transfer of GlcNAc, from UDP-GlcNAc, to endogenous phosphatidylinositol to form N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI). The GlcNAc-PI is then efficiently deacetylated to form glucosaminyl phosphatidylinositol (GlcN-PI), the substrate for subsequent reactions en route to glycolipid A.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1989 |
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| Erschienen: |
1989 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:264 |
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| Enthalten in: |
The Journal of biological chemistry - 264(1989), 19 vom: 05. Juli, Seite 11168-73 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Doering, T L [VerfasserIn] |
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| Anmerkungen: |
Date Completed 02.08.1989 Date Revised 20.03.2021 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM025069454 |
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| 100 | 1 | |a Doering, T L |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Biosynthesis of the glycosyl phosphatidylinositol membrane anchor of the trypanosome variant surface glycoprotein. Origin of the non-acetylated glucosamine |
| 264 | 1 | |c 1989 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 02.08.1989 | ||
| 500 | |a Date Revised 20.03.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Non-acetylated glucosamine is an unusual structural feature shared by all glycosyl phosphatidylinositol (GPI) lipids, including a variety of membrane anchors, the leishmanial lipophosphoglycan, and a mediator of insulin action. We proposed previously a pathway for biosynthesis of glycolipid A, the precursor of the GPI membrane anchor of the trypanosome variant surface glycoprotein (Masterson, W. J., Doering, T. L., Hart, G. W., and Englund, P. T. (1989) Cell 56, 793-800). In this paper we characterize in more detail the initial steps of GPI assembly. The first and committed step in the pathway is the transfer of GlcNAc, from UDP-GlcNAc, to endogenous phosphatidylinositol to form N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI). The GlcNAc-PI is then efficiently deacetylated to form glucosaminyl phosphatidylinositol (GlcN-PI), the substrate for subsequent reactions en route to glycolipid A | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
| 650 | 7 | |a Glycolipids |2 NLM | |
| 650 | 7 | |a Glycosylphosphatidylinositols |2 NLM | |
| 650 | 7 | |a Phosphatidylinositols |2 NLM | |
| 650 | 7 | |a Variant Surface Glycoproteins, Trypanosoma |2 NLM | |
| 650 | 7 | |a Tritium |2 NLM | |
| 650 | 7 | |a 10028-17-8 |2 NLM | |
| 650 | 7 | |a Uridine Diphosphate N-Acetylglucosamine |2 NLM | |
| 650 | 7 | |a 528-04-1 |2 NLM | |
| 650 | 7 | |a Glucosamine |2 NLM | |
| 650 | 7 | |a N08U5BOQ1K |2 NLM | |
| 650 | 7 | |a Acetylglucosamine |2 NLM | |
| 650 | 7 | |a V956696549 |2 NLM | |
| 700 | 1 | |a Masterson, W J |e verfasserin |4 aut | |
| 700 | 1 | |a Englund, P T |e verfasserin |4 aut | |
| 700 | 1 | |a Hart, G W |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:264 |g year:1989 |g number:19 |g day:05 |g month:07 |g pages:11168-73 |
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