Exploration of delta-subunit interactions in beef heart mitochondrial F1-ATPase by monoclonal antibodies
Three monoclonal antibodies (mAbs) recognizing distinct epitopes on the delta-subunit of beef heart mitochondrial F1-ATPase were studied for their reactivity towards the delta-subunit both in isolated F1 and in the F0-F1 complex of submitochondrial particles. Two of the antibodies termed mAb delta 195 and mAb delta 239 had free access to delta in F1 and the F0-F1 complex. Partial hindrance was observed for the third antibody mAb delta 22. By a double antibinding assay, it was found that the binding sites for mAb delta 195 and mAb delta 239 were close to each other and possibly overlapping. Mapping studies conducted with the isolated delta-subunit showed that mAb delta 195 and mAb delta 239 interacted with the N-terminal portion of delta extending from Ala-1 to Met-16, whereas mAb delta 22 interacted with the fragment spanning Ser-17-Glu-68. It was concluded that the Ala-1-Met-16 segment of the delta-subunit in F1 and the F0-F1 complex is freely accessible from the outside, whereas the Ser-17-Glu-68 segment of delta is partially hidden, possibly as a result of interactions with other subunits.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1989 |
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| Erschienen: |
1989 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:245 |
|---|---|
| Enthalten in: |
FEBS letters - 245(1989), 1-2 vom: 13. März, Seite 223-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lunardi, J [VerfasserIn] |
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| Themen: |
Antibodies, Monoclonal |
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| Anmerkungen: |
Date Completed 28.04.1989 Date Revised 21.06.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM024481017 |
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| 100 | 1 | |a Lunardi, J |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Exploration of delta-subunit interactions in beef heart mitochondrial F1-ATPase by monoclonal antibodies |
| 264 | 1 | |c 1989 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 28.04.1989 | ||
| 500 | |a Date Revised 21.06.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Three monoclonal antibodies (mAbs) recognizing distinct epitopes on the delta-subunit of beef heart mitochondrial F1-ATPase were studied for their reactivity towards the delta-subunit both in isolated F1 and in the F0-F1 complex of submitochondrial particles. Two of the antibodies termed mAb delta 195 and mAb delta 239 had free access to delta in F1 and the F0-F1 complex. Partial hindrance was observed for the third antibody mAb delta 22. By a double antibinding assay, it was found that the binding sites for mAb delta 195 and mAb delta 239 were close to each other and possibly overlapping. Mapping studies conducted with the isolated delta-subunit showed that mAb delta 195 and mAb delta 239 interacted with the N-terminal portion of delta extending from Ala-1 to Met-16, whereas mAb delta 22 interacted with the fragment spanning Ser-17-Glu-68. It was concluded that the Ala-1-Met-16 segment of the delta-subunit in F1 and the F0-F1 complex is freely accessible from the outside, whereas the Ser-17-Glu-68 segment of delta is partially hidden, possibly as a result of interactions with other subunits | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a Antigens |2 NLM | |
| 650 | 7 | |a Epitopes |2 NLM | |
| 650 | 7 | |a Macromolecular Substances |2 NLM | |
| 650 | 7 | |a Peptide Fragments |2 NLM | |
| 650 | 7 | |a Proton-Translocating ATPases |2 NLM | |
| 650 | 7 | |a EC 3.6.3.14 |2 NLM | |
| 650 | 7 | |a Cyanogen Bromide |2 NLM | |
| 650 | 7 | |a OS382OHJ8P |2 NLM | |
| 700 | 1 | |a Dupuis, A |e verfasserin |4 aut | |
| 700 | 1 | |a Frobert, Y |e verfasserin |4 aut | |
| 700 | 1 | |a Grassi, J |e verfasserin |4 aut | |
| 700 | 1 | |a Vignais, P V |e verfasserin |4 aut | |
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