Characterization of a monoclonal antibody (RJ5) against the immunodominant 41-kD antigen of Candida albicans
A 41-kD component of Candida albicans was identified to be the major antigen radioimmunoprecipitated by antibodies with increased titers in the sera of patients with invasive candidiasis. A mouse monoclonal antibody (RJ5) was generated which, by immunoblotting, showed positive reactivity to the immunoprecipitated 41-kD component. By two-dimensional gel electrophoresis and immunoblotting, MoAb RJ5 was shown to react with different isoforms of the 41-kD component with pI values from 6.1 to 6.9. Furthermore, MoAb RJ5 showed positive reactivity to cytoplasmic antigens of C. albicans by frozen section and immunoperoxidase staining. By SDS-polyacrylamide gel electrophoresis and immunoblotting, MoAb RJ5 showed no cross-reactivity to antigens of Candida tropicalis and Candida parapsilosis. The epitope of the 41-kD molecule recognized by MoAb RJ5 was susceptible to treatment of proteinase K at concentrations of greater than or equal to 5 micrograms/ml, and was relatively resistant to periodate oxidation with concentration of NaIO4 up to 20 mM. This MoAb may be useful in the purification and characterization of the immunodominant 41-kD antigen of C. albicans, and as a probe in the detection of Candida antigens in the sera of patients with invasive candidiasis.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1991 |
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| Erschienen: |
1991 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:96 |
|---|---|
| Enthalten in: |
International archives of allergy and applied immunology - 96(1991), 2 vom: 20., Seite 142-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shen, H D [VerfasserIn] |
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| Themen: |
Antibodies |
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| Anmerkungen: |
Date Completed 25.02.1992 Date Revised 16.08.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM017512735 |
|---|
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| 041 | |a eng | ||
| 100 | 1 | |a Shen, H D |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Characterization of a monoclonal antibody (RJ5) against the immunodominant 41-kD antigen of Candida albicans |
| 264 | 1 | |c 1991 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 25.02.1992 | ||
| 500 | |a Date Revised 16.08.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A 41-kD component of Candida albicans was identified to be the major antigen radioimmunoprecipitated by antibodies with increased titers in the sera of patients with invasive candidiasis. A mouse monoclonal antibody (RJ5) was generated which, by immunoblotting, showed positive reactivity to the immunoprecipitated 41-kD component. By two-dimensional gel electrophoresis and immunoblotting, MoAb RJ5 was shown to react with different isoforms of the 41-kD component with pI values from 6.1 to 6.9. Furthermore, MoAb RJ5 showed positive reactivity to cytoplasmic antigens of C. albicans by frozen section and immunoperoxidase staining. By SDS-polyacrylamide gel electrophoresis and immunoblotting, MoAb RJ5 showed no cross-reactivity to antigens of Candida tropicalis and Candida parapsilosis. The epitope of the 41-kD molecule recognized by MoAb RJ5 was susceptible to treatment of proteinase K at concentrations of greater than or equal to 5 micrograms/ml, and was relatively resistant to periodate oxidation with concentration of NaIO4 up to 20 mM. This MoAb may be useful in the purification and characterization of the immunodominant 41-kD antigen of C. albicans, and as a probe in the detection of Candida antigens in the sera of patients with invasive candidiasis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Antibodies |2 NLM | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a Antigens, Fungal |2 NLM | |
| 650 | 7 | |a Immunodominant Epitopes |2 NLM | |
| 700 | 1 | |a Choo, K B |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, K W |e verfasserin |4 aut | |
| 700 | 1 | |a Ling, W L |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, F C |e verfasserin |4 aut | |
| 700 | 1 | |a Han, S H |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International archives of allergy and applied immunology |d 1950 |g 96(1991), 2 vom: 20., Seite 142-8 |w (DE-627)NLM000004367 |x 0020-5915 |7 nnns |
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| 952 | |d 96 |j 1991 |e 2 |b 20 |h 142-8 | ||