Mechanistic studies of phosphoenolpyruvate carboxylase from Zea mays utilizing formate as an alternate substrate for bicarbonate
Formate is an alternate substrate for bicarbonate in the reaction with PEP catalyzed by phosphoenolpyruvate carboxylase from Zea mays, producing formyl phosphate and pyruvate. The Km for formate is 25 +/- 2 mM, and the maximum velocity is 1% of that for bicarbonate at pH 8.0. Use of [18O]formate produces inorganic phosphate containing 1 equiv of 18O, but no label is incorporated into residual phosphoenolpyruvate. PEP carboxylase catalyzes the hydrolysis of phosphoglycolate or L-phospholactate 2000 times more slowly and D-phospholactate 4000 times more slowly than the reaction between bicarbonate and PEP.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1992 |
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| Erschienen: |
1992 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:31 |
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| Enthalten in: |
Biochemistry - 31(1992), 28 vom: 21. Juli, Seite 6441-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Janc, J W [VerfasserIn] |
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| Anmerkungen: |
Date Completed 25.08.1992 Date Revised 13.06.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM013028391 |
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| 100 | 1 | |a Janc, J W |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Mechanistic studies of phosphoenolpyruvate carboxylase from Zea mays utilizing formate as an alternate substrate for bicarbonate |
| 264 | 1 | |c 1992 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 25.08.1992 | ||
| 500 | |a Date Revised 13.06.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Formate is an alternate substrate for bicarbonate in the reaction with PEP catalyzed by phosphoenolpyruvate carboxylase from Zea mays, producing formyl phosphate and pyruvate. The Km for formate is 25 +/- 2 mM, and the maximum velocity is 1% of that for bicarbonate at pH 8.0. Use of [18O]formate produces inorganic phosphate containing 1 equiv of 18O, but no label is incorporated into residual phosphoenolpyruvate. PEP carboxylase catalyzes the hydrolysis of phosphoglycolate or L-phospholactate 2000 times more slowly and D-phospholactate 4000 times more slowly than the reaction between bicarbonate and PEP | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
| 650 | 7 | |a Bicarbonates |2 NLM | |
| 650 | 7 | |a Formates |2 NLM | |
| 650 | 7 | |a Glycolates |2 NLM | |
| 650 | 7 | |a Oxygen Isotopes |2 NLM | |
| 650 | 7 | |a Pyruvates |2 NLM | |
| 650 | 7 | |a bromopyruvate |2 NLM | |
| 650 | 7 | |a 63JMV04GRK |2 NLM | |
| 650 | 7 | |a Phosphoric Monoester Hydrolases |2 NLM | |
| 650 | 7 | |a EC 3.1.3.2 |2 NLM | |
| 650 | 7 | |a Phosphoenolpyruvate Carboxylase |2 NLM | |
| 650 | 7 | |a EC 4.1.1.31 |2 NLM | |
| 650 | 7 | |a phosphoglycolate |2 NLM | |
| 650 | 7 | |a H8593JOP13 |2 NLM | |
| 700 | 1 | |a Cleland, W W |e verfasserin |4 aut | |
| 700 | 1 | |a O'Leary, M H |e verfasserin |4 aut | |
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