A regulatory subunit of the cAMP-dependent protein kinase down-regulated in aplysia sensory neurons during long-term sensitization
Binding of cAMP by the five neuronal isoforms (N1-5) of the regulatory (R) subunit of the Aplysia cAMP-dependent protein kinase is diminished in sensory neurons stimulated to produce long-term presynaptic facilitation. To determine how the cAMP-binding activity of the R subunits is lost, we isolated cDNAs encoding N4, which is a homolog of mammalian RI. Immunoblots with antisera raised against the R protein overexpressed in E. coli show that the diminished binding activity, which occurs in long-term facilitation, results from coordinate loss of R protein isoforms. No change was detected in the amount of transcripts for R subunits, suggesting that the down-regulation results from enhanced proteolytic turnover.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1992 |
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| Erschienen: |
1992 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
|---|---|
| Enthalten in: |
Neuron - 8(1992), 2 vom: 22. Feb., Seite 387-97 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bergold, P J [VerfasserIn] |
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| Themen: |
9007-49-2 |
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| Anmerkungen: |
Date Completed 26.03.1992 Date Revised 19.08.2019 published: Print GENBANK: X62382 Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM012918598 |
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| 100 | 1 | |a Bergold, P J |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A regulatory subunit of the cAMP-dependent protein kinase down-regulated in aplysia sensory neurons during long-term sensitization |
| 264 | 1 | |c 1992 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a Date Completed 26.03.1992 | ||
| 500 | |a Date Revised 19.08.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a GENBANK: X62382 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Binding of cAMP by the five neuronal isoforms (N1-5) of the regulatory (R) subunit of the Aplysia cAMP-dependent protein kinase is diminished in sensory neurons stimulated to produce long-term presynaptic facilitation. To determine how the cAMP-binding activity of the R subunits is lost, we isolated cDNAs encoding N4, which is a homolog of mammalian RI. Immunoblots with antisera raised against the R protein overexpressed in E. coli show that the diminished binding activity, which occurs in long-term facilitation, results from coordinate loss of R protein isoforms. No change was detected in the amount of transcripts for R subunits, suggesting that the down-regulation results from enhanced proteolytic turnover | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
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| 700 | 1 | |a Beushausen, S A |e verfasserin |4 aut | |
| 700 | 1 | |a Sacktor, T C |e verfasserin |4 aut | |
| 700 | 1 | |a Cheley, S |e verfasserin |4 aut | |
| 700 | 1 | |a Bayley, H |e verfasserin |4 aut | |
| 700 | 1 | |a Schwartz, J H |e verfasserin |4 aut | |
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