Evaluation of ascorbic acid 2-O-alpha-glucoside as vitamin C source : mode of intestinal hydrolysis and absorption following oral administration
Ascorbic acid 2-O-alpha-glucoside (AA-2G) has been reported to have antiscorbutic activity in guinea pigs. The present experiments examined the metabolic fate of AA-2G following ingestion. Oral administration of AA-2G (96 mg) to guinea pigs resulted in a remarkable increase of ascorbic acid in various tissues as well as plasma, but intact AA-2G was detected only in plasma, but intact AA-2G was detected only in plasma and urine in small amounts. The absorption efficiency of AA-2G and ascorbic acid was further determined by using everted gut sacs of rats. Ascorbic acid released from AA-2G on the mucosal side was effectively taken up across intestinal membranes into the serosal side, whereas AA-2G poorly permeated via a passive transport system. The hydrolysis of AA-2G on the mucosal surface of everted gut was completely inhibited by an alpha-glucosidase inhibitor and the hydrolytic activity of a crude membrane extract diminished to one-forth after immunoprecipitation with the antibody specific to maltase. From these results, it is concluded that ingested AA-2G serves as a vitamin C source through the hydrolysis by intestinal membrane-bound alpha-glucosidase, mainly maltase, and the subsequent absorption of released ascorbic acid.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1992 |
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| Erschienen: |
1992 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:62 |
|---|---|
| Enthalten in: |
International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition - 62(1992), 4 vom: 20., Seite 318-23 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Muto, N [VerfasserIn] |
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| Themen: |
2V52R0NHXW |
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| Anmerkungen: |
Date Completed 30.03.1993 Date Revised 30.11.2018 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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NLM012723371 |
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| 100 | 1 | |a Muto, N |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Evaluation of ascorbic acid 2-O-alpha-glucoside as vitamin C source |b mode of intestinal hydrolysis and absorption following oral administration |
| 264 | 1 | |c 1992 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
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| 500 | |a Date Completed 30.03.1993 | ||
| 500 | |a Date Revised 30.11.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Ascorbic acid 2-O-alpha-glucoside (AA-2G) has been reported to have antiscorbutic activity in guinea pigs. The present experiments examined the metabolic fate of AA-2G following ingestion. Oral administration of AA-2G (96 mg) to guinea pigs resulted in a remarkable increase of ascorbic acid in various tissues as well as plasma, but intact AA-2G was detected only in plasma, but intact AA-2G was detected only in plasma and urine in small amounts. The absorption efficiency of AA-2G and ascorbic acid was further determined by using everted gut sacs of rats. Ascorbic acid released from AA-2G on the mucosal side was effectively taken up across intestinal membranes into the serosal side, whereas AA-2G poorly permeated via a passive transport system. The hydrolysis of AA-2G on the mucosal surface of everted gut was completely inhibited by an alpha-glucosidase inhibitor and the hydrolytic activity of a crude membrane extract diminished to one-forth after immunoprecipitation with the antibody specific to maltase. From these results, it is concluded that ingested AA-2G serves as a vitamin C source through the hydrolysis by intestinal membrane-bound alpha-glucosidase, mainly maltase, and the subsequent absorption of released ascorbic acid | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 700 | 1 | |a Yamamoto, I |e verfasserin |4 aut | |
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