Detection of hepatitis C virus antibodies with new recombinant antigens : assessment in chronic liver diseases
A new serological assay to detect antibodies against hepatitis C, based on a recombinant protein (BHC10) which incorporates structural and non-structural viral antigens, was tested in 67 healthy subjects and 409 patients with various forms of liver disease. Results were compared with the current assay based on the recombinant non-structural viral antigen c100 and with the recently introduced second-generation assay, Ortho2. None of the healthy subjects was positive by any of the assays. In patients with chronic non-A, non-B hepatitis the prevalence of anti-BHC10 was 96.8%, higher than anti-c100 (83.3%, p less than 0.001) and similar to Ortho2 (94.3%). False-positive results were less frequently found when BHC10 was used. These findings show that assays incorporating structural and non-structural antigens provide higher sensitivity to detect hepatitis C virus infection and they define an almost exclusive role of hepatitis C virus in the genesis of chronic non-A, non-B hepatitis.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1992 |
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| Erschienen: |
1992 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Journal of hepatology - 15(1992), 3 vom: 15. Juli, Seite 309-13 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Riezu-Boj, J I [VerfasserIn] |
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| Themen: |
Antigens, Viral |
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| Anmerkungen: |
Date Completed 30.12.1992 Date Revised 17.08.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM012604380 |
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| 100 | 1 | |a Riezu-Boj, J I |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Detection of hepatitis C virus antibodies with new recombinant antigens |b assessment in chronic liver diseases |
| 264 | 1 | |c 1992 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 30.12.1992 | ||
| 500 | |a Date Revised 17.08.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A new serological assay to detect antibodies against hepatitis C, based on a recombinant protein (BHC10) which incorporates structural and non-structural viral antigens, was tested in 67 healthy subjects and 409 patients with various forms of liver disease. Results were compared with the current assay based on the recombinant non-structural viral antigen c100 and with the recently introduced second-generation assay, Ortho2. None of the healthy subjects was positive by any of the assays. In patients with chronic non-A, non-B hepatitis the prevalence of anti-BHC10 was 96.8%, higher than anti-c100 (83.3%, p less than 0.001) and similar to Ortho2 (94.3%). False-positive results were less frequently found when BHC10 was used. These findings show that assays incorporating structural and non-structural antigens provide higher sensitivity to detect hepatitis C virus infection and they define an almost exclusive role of hepatitis C virus in the genesis of chronic non-A, non-B hepatitis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Antigens, Viral |2 NLM | |
| 650 | 7 | |a DNA, Viral |2 NLM | |
| 650 | 7 | |a Hepatitis Antibodies |2 NLM | |
| 650 | 7 | |a Hepatitis C Antibodies |2 NLM | |
| 650 | 7 | |a Hepatitis C Antigens |2 NLM | |
| 650 | 7 | |a Recombinant Proteins |2 NLM | |
| 700 | 1 | |a Parker, D |e verfasserin |4 aut | |
| 700 | 1 | |a Civeira, M P |e verfasserin |4 aut | |
| 700 | 1 | |a Phippard, D |e verfasserin |4 aut | |
| 700 | 1 | |a Corbishley, T P |e verfasserin |4 aut | |
| 700 | 1 | |a Camps, J |e verfasserin |4 aut | |
| 700 | 1 | |a Castilla, A |e verfasserin |4 aut | |
| 700 | 1 | |a Prieto, J |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of hepatology |d 1993 |g 15(1992), 3 vom: 15. Juli, Seite 309-13 |w (DE-627)NLM012604372 |x 1600-0641 |7 nnns |
| 773 | 1 | 8 | |g volume:15 |g year:1992 |g number:3 |g day:15 |g month:07 |g pages:309-13 |
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| 952 | |d 15 |j 1992 |e 3 |b 15 |c 07 |h 309-13 | ||