Alpha- and beta-adrenergic receptors of the rat salivary gland. Elevation after chemical sympathectomy
Binding of [3H]dihydroergokryptine and [3H]dihydroalprenolol to membrane preparations from rat submaxillary gland was measured to characterize the alpha- and beta-adrenergic receptors, respectively. Kinetic analysis of the data revealed a high affinity binding site for each radioligand. Inhibition of binding at each site was stereospecific for the active isomer of the catecholamine used. The greater ability of a beta1 than beta2 specific beta-adrenergic antagonist to displace [3H]dihydroalprenolol binding indicated that this binding site was of the beta1 type. Chemical sympathectomy with reserpine or 6-hydroxydopamine resulted in a significant increase in both [3H]dihydroalprenolol and [3H]dihydroergokryptine binding in the rat submaxillary gland. 3scatchard analysis of the data indicated that these increases in binding were due to a change in total number of binding sites for [3H]dihydroergokryptine and [3H]dihydroalprenolol with little change in apparent affinities. This suggests that changes in alpha- and beta-adrenergic receptor density may be important in the development of supersensitivity in salivary glands after reserpine and 6-hydroxydopamine treatment.
Medienart: |
Artikel |
---|
Erscheinungsjahr: |
1979 |
---|---|
Erschienen: |
1979 |
Enthalten in: |
Zur Gesamtaufnahme - volume:584 |
---|---|
Enthalten in: |
Biochimica et biophysica acta - 584(1979), 2 vom: 01. Mai, Seite 231-41 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Pointon, S E [VerfasserIn] |
---|
Themen: |
8B1QWR724A |
---|
Anmerkungen: |
Date Completed 28.07.1979 Date Revised 09.06.2019 published: Print Citation Status MEDLINE |
---|
Förderinstitution / Projekttitel: |
|
---|
PPN (Katalog-ID): |
NLM002185563 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM002185563 | ||
003 | DE-627 | ||
005 | 20231221015448.0 | ||
007 | tu | ||
008 | 231221s1979 xx ||||| 00| ||eng c | ||
028 | 5 | 2 | |a pubmed24n0008.xml |
035 | |a (DE-627)NLM002185563 | ||
035 | |a (NLM)219900 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Pointon, S E |e verfasserin |4 aut | |
245 | 1 | 0 | |a Alpha- and beta-adrenergic receptors of the rat salivary gland. Elevation after chemical sympathectomy |
264 | 1 | |c 1979 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Completed 28.07.1979 | ||
500 | |a Date Revised 09.06.2019 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Binding of [3H]dihydroergokryptine and [3H]dihydroalprenolol to membrane preparations from rat submaxillary gland was measured to characterize the alpha- and beta-adrenergic receptors, respectively. Kinetic analysis of the data revealed a high affinity binding site for each radioligand. Inhibition of binding at each site was stereospecific for the active isomer of the catecholamine used. The greater ability of a beta1 than beta2 specific beta-adrenergic antagonist to displace [3H]dihydroalprenolol binding indicated that this binding site was of the beta1 type. Chemical sympathectomy with reserpine or 6-hydroxydopamine resulted in a significant increase in both [3H]dihydroalprenolol and [3H]dihydroergokryptine binding in the rat submaxillary gland. 3scatchard analysis of the data indicated that these increases in binding were due to a change in total number of binding sites for [3H]dihydroergokryptine and [3H]dihydroalprenolol with little change in apparent affinities. This suggests that changes in alpha- and beta-adrenergic receptor density may be important in the development of supersensitivity in salivary glands after reserpine and 6-hydroxydopamine treatment | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
650 | 7 | |a Hydroxydopamines |2 NLM | |
650 | 7 | |a Receptors, Adrenergic |2 NLM | |
650 | 7 | |a Receptors, Adrenergic, alpha |2 NLM | |
650 | 7 | |a Receptors, Adrenergic, beta |2 NLM | |
650 | 7 | |a Reserpine |2 NLM | |
650 | 7 | |a 8B1QWR724A |2 NLM | |
650 | 7 | |a Norepinephrine |2 NLM | |
650 | 7 | |a X4W3ENH1CV |2 NLM | |
700 | 1 | |a Banerjee, S P |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Biochimica et biophysica acta |d 1948 |g 584(1979), 2 vom: 01. Mai, Seite 231-41 |w (DE-627)NLM000000698 |x 0006-3002 |7 nnns |
773 | 1 | 8 | |g volume:584 |g year:1979 |g number:2 |g day:01 |g month:05 |g pages:231-41 |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 584 |j 1979 |e 2 |b 01 |c 05 |h 231-41 |