Human polymorphonuclear leucocyte migration inhibitory factor. Evidence for antigen dependency
Sudies were performed on human polymorphonuclear leucocyte migration inhibitory factor (PMN-MIF) to determine its antigen dependence. PMN-MIF produced by lymphocytes in response to purified protein derivative or coccidiodin was measured in an agarose gel system with buffy coat leucocytes as indicator cells. PMN-MIF activity contained in the lymphocyte supernatants uniformly disappeared when the supernatants were diluted 1:50 with medium; the inhibitory activity was only restored when the diluted supernatants were reconstituted with specific antigen. PMN-MIF isolated by polyacrylamide gel electrophoresis showed the same properties as PMN-MIF present in whole supernatants. This factor consistently migrated in the albumin region on gel electrophoresis. These data indicate that human PMN-MIF is antigen-dependent.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1975 |
|---|---|
| Erschienen: |
1975 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:29 |
|---|---|
| Enthalten in: |
Immunology - 29(1975), 2 vom: 15. Aug., Seite 223-30 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Weisbart, R H [VerfasserIn] |
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| Themen: |
12622-73-0 |
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| Anmerkungen: |
Date Completed 04.12.1975 Date Revised 13.11.2018 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM000524565 |
|---|
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| 100 | 1 | |a Weisbart, R H |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Human polymorphonuclear leucocyte migration inhibitory factor. Evidence for antigen dependency |
| 264 | 1 | |c 1975 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 04.12.1975 | ||
| 500 | |a Date Revised 13.11.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Sudies were performed on human polymorphonuclear leucocyte migration inhibitory factor (PMN-MIF) to determine its antigen dependence. PMN-MIF produced by lymphocytes in response to purified protein derivative or coccidiodin was measured in an agarose gel system with buffy coat leucocytes as indicator cells. PMN-MIF activity contained in the lymphocyte supernatants uniformly disappeared when the supernatants were diluted 1:50 with medium; the inhibitory activity was only restored when the diluted supernatants were reconstituted with specific antigen. PMN-MIF isolated by polyacrylamide gel electrophoresis showed the same properties as PMN-MIF present in whole supernatants. This factor consistently migrated in the albumin region on gel electrophoresis. These data indicate that human PMN-MIF is antigen-dependent | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
| 650 | 7 | |a Antigens |2 NLM | |
| 650 | 7 | |a Epitopes |2 NLM | |
| 650 | 7 | |a Macrophage Migration-Inhibitory Factors |2 NLM | |
| 650 | 7 | |a Tuberculin |2 NLM | |
| 650 | 7 | |a Coccidioidin |2 NLM | |
| 650 | 7 | |a 12622-73-0 |2 NLM | |
| 700 | 1 | |a Isaacson, J |e verfasserin |4 aut | |
| 700 | 1 | |a Bluestone, R |e verfasserin |4 aut | |
| 700 | 1 | |a Goldberg, L S |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Immunology |d 1958 |g 29(1975), 2 vom: 15. Aug., Seite 223-30 |w (DE-627)NLM000004219 |x 1365-2567 |7 nnns |
| 773 | 1 | 8 | |g volume:29 |g year:1975 |g number:2 |g day:15 |g month:08 |g pages:223-30 |
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