Degradation of polysomes of skeletal muscles of rats during preservation in solutions with different pH
Alterations in properties of RNP-particles from rat sceletal muscles on storage of the preparations in buffer solutions at various pH were analyzed by means of centrifugation in sucrose gradients. Within 3 days of incubation at 20 the distribution of RNP-particles in the sucrose gradient was changed relatively slightly; which was due to the low activity of endogenous ribosomal RNAase. The fraction of the UV-absorbing material in polysomes was gradually decreased on storage and the fraction of subunits was increased respectively. The most distinct alterations were observed on incubation of the preparations at pH 6.0; the RNP-particles were apparently destructed after acidification of the medium.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
1976 |
|---|---|
| Erschienen: |
1976 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
|---|---|
| Enthalten in: |
Voprosy meditsinskoi khimii - 22(1976), 6 vom: 01. Nov., Seite 815-9 |
| Sprache: |
Russisch |
|---|
| Weiterer Titel: |
Degradatsiia polisom skeletnykh myshts krys pri khranenii v rastvorakh s razlichnym pH |
|---|
| Beteiligte Personen: |
Kitavtsev, B A [VerfasserIn] |
|---|
| Themen: |
EC 3.1.- |
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| Anmerkungen: |
Date Completed 29.07.1977 Date Revised 15.11.2006 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM000180726 |
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| 100 | 1 | |a Kitavtsev, B A |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Degradation of polysomes of skeletal muscles of rats during preservation in solutions with different pH |
| 246 | 3 | 3 | |a Degradatsiia polisom skeletnykh myshts krys pri khranenii v rastvorakh s razlichnym pH |
| 264 | 1 | |c 1976 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 29.07.1977 | ||
| 500 | |a Date Revised 15.11.2006 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Alterations in properties of RNP-particles from rat sceletal muscles on storage of the preparations in buffer solutions at various pH were analyzed by means of centrifugation in sucrose gradients. Within 3 days of incubation at 20 the distribution of RNP-particles in the sucrose gradient was changed relatively slightly; which was due to the low activity of endogenous ribosomal RNAase. The fraction of the UV-absorbing material in polysomes was gradually decreased on storage and the fraction of subunits was increased respectively. The most distinct alterations were observed on incubation of the preparations at pH 6.0; the RNP-particles were apparently destructed after acidification of the medium | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Ribonucleoproteins |2 NLM | |
| 650 | 7 | |a Ribonucleases |2 NLM | |
| 650 | 7 | |a EC 3.1.- |2 NLM | |
| 700 | 1 | |a Shishkin, S S |e verfasserin |4 aut | |
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