Measurement of 25-hydroxyvitamin D3 in serum
We describe a method for measuring 25-hydroxyvitamin D3 in serum. Extraction with dichloromethane/methanol (2/1 by vol), followed by chromatography on a column of Sephadex LH-20, resulted in an overall analytical recovery of 82% +/- 3.5% (SD). Diluted normal rat serum was used as binding protein because it contains a transport protein that has both a high affinity (Ka = 2 X 10(10) liter/mol) and a high capacity (3 X 10(-6) mol/liter) for 25-hydroxyvitamin D3. There is no advantage in using more complex binding proteins derived either from rachitic animals or from cytosol preparations. Concentrations of 25-hydroxyvitamin D3 (13.4 +/- 4 mug/liter) in the serum of apparently normal Belgian subjects are lower than those reported for North Americans, but resemble those reported for the United Kingdom.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
1976 |
|---|---|
| Erschienen: |
1976 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
|---|---|
| Enthalten in: |
Clinical chemistry - 22(1976), 3 vom: 24. März, Seite 364-8 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Bouillon, R [VerfasserIn] |
|---|
| Themen: |
|---|
| Anmerkungen: |
Date Completed 02.06.1976 Date Revised 06.06.2023 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM000039055 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM000039055 | ||
| 003 | DE-627 | ||
| 005 | 20231221010007.0 | ||
| 007 | tu | ||
| 008 | 231221s1976 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0001.xml |
| 035 | |a (DE-627)NLM000039055 | ||
| 035 | |a (NLM)3295 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Bouillon, R |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Measurement of 25-hydroxyvitamin D3 in serum |
| 264 | 1 | |c 1976 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 02.06.1976 | ||
| 500 | |a Date Revised 06.06.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a We describe a method for measuring 25-hydroxyvitamin D3 in serum. Extraction with dichloromethane/methanol (2/1 by vol), followed by chromatography on a column of Sephadex LH-20, resulted in an overall analytical recovery of 82% +/- 3.5% (SD). Diluted normal rat serum was used as binding protein because it contains a transport protein that has both a high affinity (Ka = 2 X 10(10) liter/mol) and a high capacity (3 X 10(-6) mol/liter) for 25-hydroxyvitamin D3. There is no advantage in using more complex binding proteins derived either from rachitic animals or from cytosol preparations. Concentrations of 25-hydroxyvitamin D3 (13.4 +/- 4 mug/liter) in the serum of apparently normal Belgian subjects are lower than those reported for North Americans, but resemble those reported for the United Kingdom | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Carrier Proteins |2 NLM | |
| 650 | 7 | |a Hydroxycholecalciferols |2 NLM | |
| 700 | 1 | |a Kerkhove, P V |e verfasserin |4 aut | |
| 700 | 1 | |a De Moor, P |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Clinical chemistry |d 1955 |g 22(1976), 3 vom: 24. März, Seite 364-8 |w (DE-627)NLM000002003 |x 1530-8561 |7 nnns |
| 773 | 1 | 8 | |g volume:22 |g year:1976 |g number:3 |g day:24 |g month:03 |g pages:364-8 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 22 |j 1976 |e 3 |b 24 |c 03 |h 364-8 | ||