Genetic diversity in the coconut lethal yellowing disease phytoplasmas of East Africa
DNA primers, based on the ribosomal sequences of lethal yellowing-type disease (LYD) phytoplasmas, were used to analyse genetic variation within the lethal yellowing-type diseases of coconut in East Africa. Samples were collected from palms in Kenya, Mozambique and high, medium and low disease incidence areas of Tanzania. The mollicute-specific primer pair P1 and P6 amplified a 1.5 kbp product from all diseased palms and no product from symptomless palms, indicating that phytoplasmas were associated with all of these diseases. However, the Rohde forward and Rohde reverse primers (a second rRNA primer pair designed to detect East African LYD-associated phytoplasmas) only amplified products from Tanzanian and Kenyan diseased palms and not from those of Mozambique. Conversely, primers Ghana 813 and AK-SR, designed for specific detection of coconut-associated phytoplasmas in West Africa, amplified products only from the Mozambique palms, indicating that the phytoplasma associated with LYD in Mozambique is more closely related to those from West Africa. This was supported by restriction enzyme digestion of PCR products. DNA sequencing of PCR products from phytoplasmas within Tanzania revealed no detectable differences in the rDNA sequences of isolates from high, medium and low incidence areas..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
1999 |
|---|---|
| Erschienen: |
Oxford, U.K. and Cambridge, USA: Blackwell Publishing Ltd ; 1999 |
| Reproduktion: |
2002 ; Blackwell Publishing Journal Backfiles 1879-2005 |
|---|---|
| Enthalten in: |
Zur Gesamtaufnahme - volume:48 |
| Enthalten in: |
Plant pathology - 48(1999), 1, Seite 0 |
| Beteiligte Personen: |
Mpunami [VerfasserIn] |
|---|
| Links: |
Volltext [Deutschlandweit zugänglich] |
|---|
| Themen: |
|---|
| Umfang: |
Online-Ressource |
|---|
| doi: |
10.1046/j.1365-3059.1999.00314.x |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLEJ243803478 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ243803478 | ||
| 003 | DE-627 | ||
| 005 | 20230505211455.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 120427s1999 xx |||||o 00| ||und c | ||
| 024 | 7 | |a 10.1046/j.1365-3059.1999.00314.x |2 doi | |
| 035 | |a (DE-627)NLEJ243803478 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 100 | 1 | |a Mpunami |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Genetic diversity in the coconut lethal yellowing disease phytoplasmas of East Africa |
| 264 | 1 | |a Oxford, U.K. and Cambridge, USA |b Blackwell Publishing Ltd |c 1999 | |
| 300 | |a Online-Ressource | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a DNA primers, based on the ribosomal sequences of lethal yellowing-type disease (LYD) phytoplasmas, were used to analyse genetic variation within the lethal yellowing-type diseases of coconut in East Africa. Samples were collected from palms in Kenya, Mozambique and high, medium and low disease incidence areas of Tanzania. The mollicute-specific primer pair P1 and P6 amplified a 1.5 kbp product from all diseased palms and no product from symptomless palms, indicating that phytoplasmas were associated with all of these diseases. However, the Rohde forward and Rohde reverse primers (a second rRNA primer pair designed to detect East African LYD-associated phytoplasmas) only amplified products from Tanzanian and Kenyan diseased palms and not from those of Mozambique. Conversely, primers Ghana 813 and AK-SR, designed for specific detection of coconut-associated phytoplasmas in West Africa, amplified products only from the Mozambique palms, indicating that the phytoplasma associated with LYD in Mozambique is more closely related to those from West Africa. This was supported by restriction enzyme digestion of PCR products. DNA sequencing of PCR products from phytoplasmas within Tanzania revealed no detectable differences in the rDNA sequences of isolates from high, medium and low incidence areas. | ||
| 533 | |d 2002 |f Blackwell Publishing Journal Backfiles 1879-2005 |7 |2002|||||||||| | ||
| 650 | 4 | |a coconut | |
| 700 | 1 | |a Tymon |e verfasserin |4 aut | |
| 700 | 1 | |a Jones |e verfasserin |4 aut | |
| 700 | 1 | |a Dickinson |4 oth | |
| 773 | 0 | 8 | |i In |t Plant pathology |d Oxford [u.a.] : Wiley-Blackwell, 1952 |g 48(1999), 1, Seite 0 |h Online-Ressource |w (DE-627)NLEJ243927657 |w (DE-600)2020845-5 |x 1365-3059 |7 nnns |
| 773 | 1 | 8 | |g volume:48 |g year:1999 |g number:1 |g pages:0 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1046/j.1365-3059.1999.00314.x |q text/html |x Verlag |z Deutschlandweit zugänglich |3 Volltext |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a ZDB-1-DJB | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 48 |j 1999 |e 1 |h 0 | ||