Determination of serum aflatoxin B1-lysine and biochemical parameters in broiler chicks fed an aflatoxin B1-contaminated diet / R. E. Rosim, D. E. Faria Filho, T. W. Almeida, C. H. Corassin, C. A. F. Oliveira
Abstract In the present trial, the levels of serum aflatoxin B 1 (AFB 1 )-lysine and their relationship with biochemical parameters in broiler chicks fed an AFB 1 -contaminated diet were determined. The experimental design was completely randomized with two treatments (control and 222.17 µg/kg AFB 1 ) and 20 bird per treatment. Feeds were offered to broiler chicks for 14 days, from 28 to 42 days of age. Animals were vaccinated against Newcastle’s and Marek’s diseases on the 14th day of life, and were killed at 42 days of age. Broilers receiving AFB 1 did not demonstrate any sign of toxicity. Compared with controls, aspartate aminotransferase and globulin levels were not affected in the AFB 1 -treated group. However, higher levels of gamma-glutamyl transferase and lower concentrations of total protein and albumin were observed in the group receiving AFB 1 on days 35 and 42. AFB 1 -lysine were detected in the serum of all broilers fed the AFB 1 -contaminated diet, at mean levels of 56.52–77.83 ng/mg albumin on days 35 and 42 of age, respectively. These values indicated the internal dose of AFB 1 in birds, which negatively correlated with total protein, albumin, and globulin levels. Data indicated that AFB 1 -lysine shows the potential to be a sensitive and specific biomarker for the evaluation of broiler exposure to dietary aflatoxin, as well as for diagnostic purposes. Further studies are necessary to determine physiologically-based toxicokinetics of serum AFB 1 -lysine in broilers.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:43 |
|---|---|
| Enthalten in: |
Drug and chemical toxicology - 43(2020), 6, Seite 623-629 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Rosim, R. E. [VerfasserIn] |
|---|
| Links: |
FID Access [lizenzpflichtig] |
|---|
| Themen: |
|---|
| Umfang: |
1 Online-Ressource (7 p) |
|---|
| doi: |
10.1080/01480545.2019.1578786 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
KFL011185058 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | KFL011185058 | ||
| 003 | DE-627 | ||
| 005 | 20231109210037.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230714s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1080/01480545.2019.1578786 |2 doi | |
| 035 | |a (DE-627)KFL011185058 | ||
| 035 | |a (KFL)prod_LgpH_10.1080/01480545.2019.1578786 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 084 | |a PHARM |q DE-84 |2 fid | ||
| 100 | 1 | |a Rosim, R. E. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Determination of serum aflatoxin B1-lysine and biochemical parameters in broiler chicks fed an aflatoxin B1-contaminated diet |c R. E. Rosim, D. E. Faria Filho, T. W. Almeida, C. H. Corassin, C. A. F. Oliveira |
| 264 | 1 | |c 2020 | |
| 300 | |a 1 Online-Ressource (7 p) | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract In the present trial, the levels of serum aflatoxin B 1 (AFB 1 )-lysine and their relationship with biochemical parameters in broiler chicks fed an AFB 1 -contaminated diet were determined. The experimental design was completely randomized with two treatments (control and 222.17 µg/kg AFB 1 ) and 20 bird per treatment. Feeds were offered to broiler chicks for 14 days, from 28 to 42 days of age. Animals were vaccinated against Newcastle’s and Marek’s diseases on the 14th day of life, and were killed at 42 days of age. Broilers receiving AFB 1 did not demonstrate any sign of toxicity. Compared with controls, aspartate aminotransferase and globulin levels were not affected in the AFB 1 -treated group. However, higher levels of gamma-glutamyl transferase and lower concentrations of total protein and albumin were observed in the group receiving AFB 1 on days 35 and 42. AFB 1 -lysine were detected in the serum of all broilers fed the AFB 1 -contaminated diet, at mean levels of 56.52–77.83 ng/mg albumin on days 35 and 42 of age, respectively. These values indicated the internal dose of AFB 1 in birds, which negatively correlated with total protein, albumin, and globulin levels. Data indicated that AFB 1 -lysine shows the potential to be a sensitive and specific biomarker for the evaluation of broiler exposure to dietary aflatoxin, as well as for diagnostic purposes. Further studies are necessary to determine physiologically-based toxicokinetics of serum AFB 1 -lysine in broilers | ||
| 653 | |a AFB1-lysine | ||
| 653 | |a biomarker | ||
| 653 | |a LC-MS/MS | ||
| 653 | |a broilers | ||
| 653 | |a toxicity | ||
| 700 | 1 | |a Faria Filho, D. E. |e verfasserin |4 aut | |
| 700 | 1 | |a Almeida, T. W. |e verfasserin |4 aut | |
| 700 | 1 | |a Corassin, C. H. |e verfasserin |4 aut | |
| 700 | 1 | |a Oliveira, C. A. F. |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Drug and chemical toxicology |d Abingdon : Taylor & Francis Group, 1977 |g 43(2020), 6, Seite 623-629 |h Online-Ressource |w (DE-627)KFL000006009 |w (DE-600)2028063-4 |w (DE-576)273879553 |x 1525-6014 |7 nnns |
| 773 | 1 | 8 | |g volume:43 |g year:2020 |g number:6 |g pages:623-629 |
| 856 | 4 | 0 | |u http://pharmazie.proxy.fid-lizenzen.de/fid/tandf-ejournals-pharmazie/doi.org/10.1080/01480545.2019.1578786 |m X:KFL |x Resolving-System |y FID Access |z lizenzpflichtig |
| 912 | |a ZDB-1-TFE | ||
| 912 | |a GBV_KFL | ||
| 912 | |a FID-PHARM | ||
| 935 | |i IMPORT_0628_prod_LgpH_02 | ||
| 951 | |a AR | ||
| 952 | |d 43 |j 2020 |e 6 |h 623-629 | ||