Metabolic Study of Benzamidenafil using Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry / Jaesung Pyo
Background: Various illegal phosphodiesterase-5 (PDE5) inhibitors have been indiscriminately added to food and dietary supplements since the early 2000s without evaluating their side effects or pharmaceutical effects. Objective: The metabolic pathway of benzamidenafil was evaluated using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-ToF-MS). Methods: In vitro metabolic samples were prepared using human liver microsomes, and in vivo metabolic samples were obtained from drug-administered rats. The metabolic samples were extracted to remove biological interferences and injected into the LC-Q-ToF-MS system for analysis. Their mass spectra and chromatograms were compared and interpreted. Results: A total of nine metabolites were evaluated in the benzamidenafil-treated samples. All error values were within 5 ppm with high accuracy. Conclusion: These newly identified metabolites of benzamidenafil could be used to construct relevant legal databases and applied to related forensic cases.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
Current pharmaceutical analysis - 18(2022), 6, Seite 6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Pyo, Jaesung [VerfasserIn] |
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| Links: |
FID Access [lizenzpflichtig] |
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| Umfang: |
1 Online-Ressource (6 p) |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
KFL011170549 |
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| 520 | |a Background: Various illegal phosphodiesterase-5 (PDE5) inhibitors have been indiscriminately added to food and dietary supplements since the early 2000s without evaluating their side effects or pharmaceutical effects. Objective: The metabolic pathway of benzamidenafil was evaluated using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-ToF-MS). Methods: In vitro metabolic samples were prepared using human liver microsomes, and in vivo metabolic samples were obtained from drug-administered rats. The metabolic samples were extracted to remove biological interferences and injected into the LC-Q-ToF-MS system for analysis. Their mass spectra and chromatograms were compared and interpreted. Results: A total of nine metabolites were evaluated in the benzamidenafil-treated samples. All error values were within 5 ppm with high accuracy. Conclusion: These newly identified metabolites of benzamidenafil could be used to construct relevant legal databases and applied to related forensic cases | ||
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