Pharmacokinetic Interaction between Asari Radix et Rhizoma and Dried Ginger (Zingiber officinalis) in Rats / Xingxing Zhuang, Li Zhou, Renhua Miao, Shoudong Ni, Meng Li

Introduction: Asari Radix et Rhizoma (ARR) and dried ginger (Zingiber officinalis) (DG) are often used together in drug preparations in traditional Chinese medicine (TCM) to treat respiratory diseases, including cold, bronchitis and pneumonia. Previous studies suggested that ARR and/or DG may influence the pharmacokinetics of other herbal components. In the current study, we examined pharmacokinetic interactions between ARR and DG in rats after oral administration. Methods: We developed a method based on ultra-high-performance liquid chromatographytandem mass spectrometry to simultaneously measure serum concentrations of two active components each in ARR (L-asarinin and sesamin) and DG (6-gingerol and 6-shogaol). Adult Sprague- Dawley rats were starved overnight, then given ARR extract, DO extract, or a co-decoction of ARR and DG by gastric gavage (6 g raw material per kg body weight; n = 6 per group). Blood samples were collected prior to drug administration and at the following times (h) afterward: 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 6.0, 8.0, 12.0 and 24.0. Pharmacokinetic parameters were compared using Student’s t test for independent samples. Results: A simple, rapid, sensitive analytical method has been developed to detect four bioactive components simultaneously in the ARR-DG herbal pair. Pharmacokinetic parameters including C max , T max , T 1/2 and AUC (0~t) were calculated using the non-compartmental model with the DAS 2.0 pharmacokinetic software. For L-asarinin, T max was 2.00 ± 0.00 h in ARR animals and 1.67±0.26 h in ARR-DG animals (P max was 350.48 ± 23.85 ng/mL in DG animals and 300.21 ± 20.02 ng/mL in ARR-DG (P max was 2.83 ± 0.41 h in DG and 2.17 ± 0.41 h in ARR-DG (P (0~t) was 1.93 ± 0.15 mg/mL.h in ARR and 1.70 ± 0.15 mg/mL.h in ARR-DG (P max was 390.28 ± 26.02 ng/mL in DG animals and 455.63 ± 31.01 ng/mL in ARR-DG (P max was 2.93 ± 0.10 h in DG and 1.92 ± 0.10 h in ARR-DG (P 1/2 was 3.74 ± 0.29 h in DG and 3.28 ± 0.22 h in ARR-DG (P (0~t) was 2.15 ± 0.18 mg/mL.h in DG and 2.73 ± 0.15 mg/mL.h in ARR-DG (P max , increased T 1/2 but did not affect the overall bioavailability of L-asarinin in ARR. The interactions in ARR-DG decreased C max and T max but increased T1/2 and AUC (0~t) of 6-gingerol in DG. The interactions increased Cmax and AUC (0~t) but decreased Tmax and T 1/2 of 6-shogaol in DG. Interactions in ARRDG did not affect the pharmacokinetics of sesamin.

Medienart:	E-Artikel

Erscheinungsjahr:	2022
Erschienen:	2022

Enthalten in:	Zur Gesamtaufnahme - volume:18
Enthalten in:	Current pharmaceutical analysis - 18(2022), 1, Seite 8

Sprache:	Englisch

Beteiligte Personen:	Zhuang, Xingxing [VerfasserIn] Zhou, Li [VerfasserIn] Miao, Renhua [VerfasserIn] Ni, Shoudong [VerfasserIn] Li, Meng [VerfasserIn]

Links:	FID Access [lizenzpflichtig]

Umfang:	1 Online-Ressource (8 p)

Förderinstitution / Projekttitel:

PPN (Katalog-ID):	KFL011169982