Pretreatment with Gallic Acid Mitigates Cyclophosphamide Induced Inflammationand Oxidative Stress in Mice / Saeed Baharmi, Heibatullah Kalantari, Mojtaba Kalantar, Mehdi Goudarzi, Esrafil Mansouri, Hadi Kalantar
Background: Cyclophosphamide (CP) as an alkylating compound has been widely appliedto treat cancer and autoimmune diseases. CP is observed to be nephrotoxic in humans and animalsbecause it produces reactive oxygen species. Gallic Acid (GA), a polyhydroxy phenolic compound,is reported to exhibit antioxidant and anti-inflammatory effects. Objective: The current research aimed at evaluating the GA effect on CP-related renal toxicity. Methods: In total, 35 male mice were assigned to 5 groups. Group1: receiving normal saline, group2: CP group, receiving one CP injection (200 mg/kg; i.p.) on day 6. Groups 3 and 4: GA+CP, GA(10 and 30 mg/kg; p.o.; respectively) received through six consecutive days plus CP on the 6th day2 hr after the last dose of GA, group 5: received GA (30 mg/kg; p.o.) for six consecutive days.Then on day 7, blood samples were collected for determining Creatinine (Cr), serum kidney injurymolecule-1 (KIM-1), Blood Urea Nitrogen (BUN), and Neutrophil Gelatinase-Associated Lipocalin(NGAL) concentrations. Malondialdehyde (MDA), Nitric Oxide (NO) concentration, Catalase(CAT), Superoxide Dismutase (SOD), Glutathione (GSH), Glutathione Peroxidase (GPx) activities,and IL-1β, TNF-α levels were assessed in renal tissue. Results: CP administration significantly increases KIM-1, NGAL, Cr, BUN, MDA, NO, IL-1β,and TNF-α level. It also decreases GSH concentration, SOD, GPx, and CAT function. Pretreatmentwith GA prevented these changes. Histopathological assessments approved the GA protective effect. Conclusion: Our results showed that GA is possibly effective as a protective agent in cyclophosphamide-associated toxicities.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2022 |
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Erschienen: |
2022 |
Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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Enthalten in: |
Current molecular pharmacology - 15(2022), 1, Seite 9 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Baharmi, Saeed [VerfasserIn] |
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Links: |
FID Access [lizenzpflichtig] |
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Umfang: |
1 Online-Ressource (9 p) |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
KFL01116817X |
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520 | |a Background: Cyclophosphamide (CP) as an alkylating compound has been widely appliedto treat cancer and autoimmune diseases. CP is observed to be nephrotoxic in humans and animalsbecause it produces reactive oxygen species. Gallic Acid (GA), a polyhydroxy phenolic compound,is reported to exhibit antioxidant and anti-inflammatory effects. Objective: The current research aimed at evaluating the GA effect on CP-related renal toxicity. Methods: In total, 35 male mice were assigned to 5 groups. Group1: receiving normal saline, group2: CP group, receiving one CP injection (200 mg/kg; i.p.) on day 6. Groups 3 and 4: GA+CP, GA(10 and 30 mg/kg; p.o.; respectively) received through six consecutive days plus CP on the 6th day2 hr after the last dose of GA, group 5: received GA (30 mg/kg; p.o.) for six consecutive days.Then on day 7, blood samples were collected for determining Creatinine (Cr), serum kidney injurymolecule-1 (KIM-1), Blood Urea Nitrogen (BUN), and Neutrophil Gelatinase-Associated Lipocalin(NGAL) concentrations. Malondialdehyde (MDA), Nitric Oxide (NO) concentration, Catalase(CAT), Superoxide Dismutase (SOD), Glutathione (GSH), Glutathione Peroxidase (GPx) activities,and IL-1β, TNF-α levels were assessed in renal tissue. Results: CP administration significantly increases KIM-1, NGAL, Cr, BUN, MDA, NO, IL-1β,and TNF-α level. It also decreases GSH concentration, SOD, GPx, and CAT function. Pretreatmentwith GA prevented these changes. Histopathological assessments approved the GA protective effect. Conclusion: Our results showed that GA is possibly effective as a protective agent in cyclophosphamide-associated toxicities | ||
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