Assessment of a Novel Vitamin D3Formulation with Nanostructured Lipid Carriers for Transdermal Delivery / Laura Junqueira, Hudson Polonini, Cristiano Ramos, Anderson O. Ferreira, Nádia Raposo, Marcos Brandão
Objective: Develop and assess a transdermal emulsion loaded with nanostructured lipid carriers for vitamin D 3 supplementation. Methods: Vitamin D 3 loaded nanostructured lipid carriers, produced via high shear homogenization and ultrasonication, were assessed for their particle size, distribution, morphology, zeta potential, entrapment efficiency, and cytotoxicity. They were incorporated into a transdermal vehicle, and the stability and ex vivo permeation were evaluated. Results: Spherical nanoparticles were developed with a particle size of 192.5 nm, a polydispersity index of 0.13, a zeta potential of -29.0 mV, and an entrapment efficiency of 99.75%. They were stable (particle size and distribution) for 15 days when stored in a refrigerator, and for 30 days at room temperature and 32°C. The nanoparticles decreased the drug cytotoxicity against fibroblasts, as shown by IC 50 (nanoparticle: 32.48 μg mL −1 ; vitamin D 3 : 16.73 μg mL −1 ). The emulsion loaded with nanoparticles minimized the degradation of vitamin D3 when compared with the nanoparticle dispersion. Additionally, the emulsion provided the skin permeation of vitamin D3 following the recommended daily allowance. Conclusion: To the best of our knowledge, this is the first study to use nanostructured lipid carriers for transdermal delivery of vitamin D. The developed formulation is a promising strategy to overcome the vitamin D 3 variable oral bioavailability. It also represents a comfortable route of administration; thus it could be beneficial for patients and clinicians. However, further studies are needed to allow the permeation of larger amounts of vitamin D3, and the combination of these nanoparticles with microneedles would be interesting.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:19 |
|---|---|
| Enthalten in: |
Current drug delivery - 19(2022), 5, Seite 11 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Junqueira, Laura [VerfasserIn] |
|---|
| Links: |
FID Access [lizenzpflichtig] |
|---|
| Umfang: |
1 Online-Ressource (11 p) |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
KFL011160039 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | KFL011160039 | ||
| 003 | DE-627 | ||
| 005 | 20231128080709.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230613s2022 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)KFL011160039 | ||
| 035 | |a (KFL)prod_DARH_.7728E1D57AB712574DE040D5B99E1ACACFFE3764 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 084 | |a PHARM |q DE-84 |2 fid | ||
| 100 | 1 | |a Junqueira, Laura |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Assessment of a Novel Vitamin D3Formulation with Nanostructured Lipid Carriers for Transdermal Delivery |c Laura Junqueira, Hudson Polonini, Cristiano Ramos, Anderson O. Ferreira, Nádia Raposo, Marcos Brandão |
| 264 | 1 | |c 2022 | |
| 300 | |a 1 Online-Ressource (11 p) | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Objective: Develop and assess a transdermal emulsion loaded with nanostructured lipid carriers for vitamin D 3 supplementation. Methods: Vitamin D 3 loaded nanostructured lipid carriers, produced via high shear homogenization and ultrasonication, were assessed for their particle size, distribution, morphology, zeta potential, entrapment efficiency, and cytotoxicity. They were incorporated into a transdermal vehicle, and the stability and ex vivo permeation were evaluated. Results: Spherical nanoparticles were developed with a particle size of 192.5 nm, a polydispersity index of 0.13, a zeta potential of -29.0 mV, and an entrapment efficiency of 99.75%. They were stable (particle size and distribution) for 15 days when stored in a refrigerator, and for 30 days at room temperature and 32°C. The nanoparticles decreased the drug cytotoxicity against fibroblasts, as shown by IC 50 (nanoparticle: 32.48 μg mL −1 ; vitamin D 3 : 16.73 μg mL −1 ). The emulsion loaded with nanoparticles minimized the degradation of vitamin D3 when compared with the nanoparticle dispersion. Additionally, the emulsion provided the skin permeation of vitamin D3 following the recommended daily allowance. Conclusion: To the best of our knowledge, this is the first study to use nanostructured lipid carriers for transdermal delivery of vitamin D. The developed formulation is a promising strategy to overcome the vitamin D 3 variable oral bioavailability. It also represents a comfortable route of administration; thus it could be beneficial for patients and clinicians. However, further studies are needed to allow the permeation of larger amounts of vitamin D3, and the combination of these nanoparticles with microneedles would be interesting | ||
| 700 | 1 | |a Polonini, Hudson |e verfasserin |4 aut | |
| 700 | 1 | |a Ramos, Cristiano |e verfasserin |4 aut | |
| 700 | 1 | |a Ferreira, Anderson O. |e verfasserin |4 aut | |
| 700 | 1 | |a Raposo, Nádia |e verfasserin |4 aut | |
| 700 | 1 | |a Brandão, Marcos |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Current drug delivery |d Bussum [u.a.] : Bentham Science Publ., 2004 |g 19(2022), 5, Seite 11 |h Online-Ressource |w (DE-627)KFL000006416 |w (DE-600)2155966-1 |w (DE-576)273889680 |x 1875-5704 |7 nnns |
| 773 | 1 | 8 | |g volume:19 |g year:2022 |g number:5 |g pages:11 |
| 856 | 4 | 0 | |u http://pharmazie.proxy.fid-lizenzen.de/fid/bentham-ejournals-pharmazie/www.eurekaselect.com/openurl/content.php?genre=article&issn=1567-2018&volume=19&issue=5&spage=614 |m X:KFL |x Verlag |y FID Access |z lizenzpflichtig |
| 912 | |a ZDB-1-BEJ | ||
| 912 | |a GBV_KFL | ||
| 912 | |a FID-PHARM | ||
| 935 | |i IMPORT_0628_prod_DARH_03 | ||
| 951 | |a AR | ||
| 952 | |d 19 |j 2022 |e 5 |h 11 | ||