Effects of Xenogen Mesenchymal Stem Cells and Cryo-Platelet Gel onIntractable Wound Healing in Animal Model (Rat) / Babak Alavi-Farzaneh, Ali Shojaeian, Mehdi Banitalebi-Dehkordi, Fatemeh Mirahmadi, Ameneh Mehri-Ghahfarrokhi, Alireza Ghorbanpour, Shima Rahmati-Dehkordi, Farshad Yazdani
Background: Today, the effects of growth factors and mesenchymal stem cells (MSCs)in promoting wound healing has been confirmed. Objective: This study aimed to investigate the effect of MSCs and platelet cryogel on wound healing. Methods: 40 male wistar rats were randomly divided into five groups (n=8). The control groupwas just dressed, the second group received platelet cryogel, the third group received platelet cryogelcontaining MSCs, the fourth group received plasma, and the fifth group received plasma plusMSCs. The biopsy was obtained from the wounds in the 2, 4, 6, and 8 days of the treatment. Then,pathological evaluation was conducted. Finally, qRT-PCR was performed to determine angiogenesis. Results: The intervention groups had faster wound healing and lower wound area than the controlgroup (p<0.05). The highest wound healing rate and the smallest wound area was observed in thegroup receiving platelet cryogel plus MSCs. Angiogenesis, fibrosis, myoepithelial and epithelializationin the pathologic examination using H & E staining were not significantly different betweenthe groups. The expression of Ang-1 in the intervention groups was higher than the control groupand the highest expression was observed in the platelet cryogel plus MSCs, followed by theplatelet cryogel group. The expression of VEGF in the plasma plus MSCs was higher than in theother groups. Conclusion: Further studies require to determine the effects of combined use of platelet cryogelplus MSCs on other types of wound and evaluate mechanisms involved in wound healing like collagenesisand inflammatory factors.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
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| Enthalten in: |
Anti-inflammatory & anti-allergy agents in medicinal chemistry - 20(2021), 4, Seite 9 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Alavi-Farzaneh, Babak [VerfasserIn] |
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| Links: |
FID Access [lizenzpflichtig] |
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| Umfang: |
1 Online-Ressource (9 p) |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Background: Today, the effects of growth factors and mesenchymal stem cells (MSCs)in promoting wound healing has been confirmed. Objective: This study aimed to investigate the effect of MSCs and platelet cryogel on wound healing. Methods: 40 male wistar rats were randomly divided into five groups (n=8). The control groupwas just dressed, the second group received platelet cryogel, the third group received platelet cryogelcontaining MSCs, the fourth group received plasma, and the fifth group received plasma plusMSCs. The biopsy was obtained from the wounds in the 2, 4, 6, and 8 days of the treatment. Then,pathological evaluation was conducted. Finally, qRT-PCR was performed to determine angiogenesis. Results: The intervention groups had faster wound healing and lower wound area than the controlgroup (p<0.05). The highest wound healing rate and the smallest wound area was observed in thegroup receiving platelet cryogel plus MSCs. Angiogenesis, fibrosis, myoepithelial and epithelializationin the pathologic examination using H & E staining were not significantly different betweenthe groups. The expression of Ang-1 in the intervention groups was higher than the control groupand the highest expression was observed in the platelet cryogel plus MSCs, followed by theplatelet cryogel group. The expression of VEGF in the plasma plus MSCs was higher than in theother groups. Conclusion: Further studies require to determine the effects of combined use of platelet cryogelplus MSCs on other types of wound and evaluate mechanisms involved in wound healing like collagenesisand inflammatory factors | ||
| 700 | 1 | |a Shojaeian, Ali |e verfasserin |4 aut | |
| 700 | 1 | |a Banitalebi-Dehkordi, Mehdi |e verfasserin |4 aut | |
| 700 | 1 | |a Mirahmadi, Fatemeh |e verfasserin |4 aut | |
| 700 | 1 | |a Mehri-Ghahfarrokhi, Ameneh |e verfasserin |4 aut | |
| 700 | 1 | |a Ghorbanpour, Alireza |e verfasserin |4 aut | |
| 700 | 1 | |a Rahmati-Dehkordi, Shima |e verfasserin |4 aut | |
| 700 | 1 | |a Yazdani, Farshad |e verfasserin |4 aut | |
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