Identification of Phytoconstituents ofTragia Involucrataleaf Extracts and Evaluate their Correlation with Anti-inflammatory & Antioxidant Properties / Vinodhini Velu, Swagata Banerjee, Vidya Radhakrishnan, Gaurav Gupta, Dinesh Kumar Chellappan, Neeraj Kumar Fuloria, Shivkanya Fuloria, Meenu Mehta, Kamal Dua, Himaja Malipeddi
Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts. Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using Soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H 2 O 2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be a major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract. Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28-epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine- 1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P 2 O 2 method (73.58%) at 200 μg mL -1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study. Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent anti- inflammatory property.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
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| Enthalten in: |
Anti-inflammatory & anti-allergy agents in medicinal chemistry - 20(2021), 3, Seite 8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Velu, Vinodhini [VerfasserIn] |
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| Links: |
FID Access [lizenzpflichtig] |
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| Umfang: |
1 Online-Ressource (8 p) |
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| Förderinstitution / Projekttitel: |
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KFL011145161 |
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| 245 | 1 | 0 | |a Identification of Phytoconstituents ofTragia Involucrataleaf Extracts and Evaluate their Correlation with Anti-inflammatory & Antioxidant Properties |c Vinodhini Velu, Swagata Banerjee, Vidya Radhakrishnan, Gaurav Gupta, Dinesh Kumar Chellappan, Neeraj Kumar Fuloria, Shivkanya Fuloria, Meenu Mehta, Kamal Dua, Himaja Malipeddi |
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| 520 | |a Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts. Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using Soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H 2 O 2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be a major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract. Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28-epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine- 1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P 2 O 2 method (73.58%) at 200 μg mL -1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study. Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent anti- inflammatory property | ||
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