Effect of Plant Growth Regulators onin VitroPropagation ofParis Polyphylla / Arcadius Puwein, Shiny C. Thomas
ABSTRACT In vitro propagation using plant growth regulators (PGRs) was evaluated in Paris polyphylla . Thin cell layers (0.5–1 mm) of rhizomes (explants) were sliced and inoculated on half-strength MS (Murashige and Skoog) medium with 3% (w/v) sucrose and solidified with 0.3% (w/v) clerigel. In this medium, 50% of the explants were treated with (0.5 µg mL −1 ) activated charcoal (AC) and 50% without AC. The half strength MS medium was supplemented with different concentrations of 6-benzylaminopurine (BAP) either individually or in combinations with auxins. The optimal percentage (80%) of shoot response was induced by the synergistic action of BAP (0.5 µg mL −1 ) and α-naphthalene acetic acid (NAA) (0.2 µg mL −1 ) in the medium containing AC. After sub-culturing, the highest number (4.00 ± 1.00) of shoot buds per explant was achieved in the MS medium with AC, which was augmented with a combination of BAP (0.5 µg mL −1 ) and NAA (0.2 µg mL −1 ). The medium containing AC showed greater shoot induction than the non-AC medium. Up to 94.4% of the regenerated shoots successfully established in the greenhouse soil.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
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| Enthalten in: |
Journal of herbs, spices & medicinal plants - 28(2022), 2, Seite 206-216 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Puwein, Arcadius [VerfasserIn] |
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| Links: |
FID Access [lizenzpflichtig] |
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| Themen: |
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| Umfang: |
1 Online-Ressource (11 p) |
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| doi: |
10.1080/10496475.2022.2045416 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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KFL011137576 |
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| 520 | |a ABSTRACT In vitro propagation using plant growth regulators (PGRs) was evaluated in Paris polyphylla . Thin cell layers (0.5–1 mm) of rhizomes (explants) were sliced and inoculated on half-strength MS (Murashige and Skoog) medium with 3% (w/v) sucrose and solidified with 0.3% (w/v) clerigel. In this medium, 50% of the explants were treated with (0.5 µg mL −1 ) activated charcoal (AC) and 50% without AC. The half strength MS medium was supplemented with different concentrations of 6-benzylaminopurine (BAP) either individually or in combinations with auxins. The optimal percentage (80%) of shoot response was induced by the synergistic action of BAP (0.5 µg mL −1 ) and α-naphthalene acetic acid (NAA) (0.2 µg mL −1 ) in the medium containing AC. After sub-culturing, the highest number (4.00 ± 1.00) of shoot buds per explant was achieved in the MS medium with AC, which was augmented with a combination of BAP (0.5 µg mL −1 ) and NAA (0.2 µg mL −1 ). The medium containing AC showed greater shoot induction than the non-AC medium. Up to 94.4% of the regenerated shoots successfully established in the greenhouse soil | ||
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