Preparation and evaluation of paclitaxel-loaded reactive oxygen species and glutathione redox-responsive poly(lactic-co-glycolic acid) nanoparticles for controlled release in tumor cells / Siying Wang, Feng Han, Lanlan Xie, Yanjie Liu, Qilei Yang, Dongmei Zhao, Xiuhua Zhao

Aim:To formulate and assess the anticancer effect of the poly(lactic-co-glycolic acid) (PLGA) copolymer with the thioether groups (diethyl sulfide [Des]) and disulfide bond (cystamine containing disulfide [Cys]), which encapsulated the anticancer drug paclitaxel (PTX) and triggered PTX release in cancer cell H2O2-rich or glutathione-rich surroundings.Methods:PLGA-b-P (DesCys) and PLGA-b-P nanoparticles loaded with PTX were prepared and characterizedin vitro. The delivery ability of the PLGA-b-P nanoparticles and PLGA-b-P-PTX nanoparticles was assessed on a CT26 (mouse colon cancer cell line) and mouse lung cancer LLC model.Results:The nanoparticles were successfully prepared. Compared with free PTX, the formulated PLGA-b-P nanoparticles loaded with PTX exhibited greater accumulation at the tumor site in the mouse model.Conclusion:PLGA-b-P nanoparticles promote drug accumulation at tumor sites, providing an effective strategy for an intelligent, responsive drug-delivery system. Aim: 2 2 Methods: b b in vitro b b Results: b Conclusion: b Plain language summary Cancer is one of the diseases with the highest mortality rate in the world. Chemotherapy is the most frequently used method of cancer treatment. However, traditional chemotherapeutic drugs have the disadvantages of lack of specificity, high toxicity to the human body, lack of tumor targeting, inability to exist stably in the body, ease of clearance by the body and drug resistance to tumors, so clinical application is limited. Paclitaxel is widely used in the treatment of various cancers, and its use is limited due to the above disadvantages. The purpose of this study was to construct a nanodelivery system with dual responses to the tumor microenvironment, enabling the efficient accumulation of paclitaxel in solid tumors through the enhanced permeability and retention effect, enhancing the therapeutic effect of cancer. This study also investigated the intravenous safety of this dual-responsive nanodelivery system. It evaluated the toxic effects of drug-loaded nanoparticles on cancer cells and validated the controlled release of nanoparticles at tumor sites by in vivoimaging in mice. The authors concluded that tumor microenvironment dual-responsive nanoparticles are a novel and promising nanodelivery system as an intelligently responsive anticancer agent with great promise in future clinical trials. in vivo Tweetable abstract This study prepared polymer nanocarriers (PLGA-b-p-NPs) with double response to the tumor microenvironment and loaded with traditional antitumor drug paclitaxel, so that drugs can accurately reach tumor cells and be rapidly released in cells. b.

Medienart:	E-Artikel

Erscheinungsjahr:	2023
Erschienen:	Erscheinungsort nicht ermittelbar: 2023

Enthalten in:	Nanomedicine

Sprache:	Englisch

Beteiligte Personen:	Wang, Siying [VerfasserIn] Han, Feng [VerfasserIn] Xie, Lanlan [VerfasserIn] Liu, Yanjie [VerfasserIn] Yang, Qilei [VerfasserIn] Zhao, Dongmei [VerfasserIn] Zhao, Xiuhua [VerfasserIn]

Links:	FID Access [lizenzpflichtig]

Themen:	Disulfide bond Dual-responsive nanoparticles GSH-sensitive PLGA Paclitaxel Polymer nanoparticles ROS-sensitive Targeted delivery Thioether bond Tumor microenvironment

Umfang:	1 Online-Ressource (22 p)

doi:	10.2217/nnm-2022-0164

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	KFL011095954