Hepatotoxicity of copper sulfide nanoparticles towards hepatocyte spheroids using a novel multi-concave agarose chip method / Tianyan Jiang, Haoxiang Guo, Ya-Nan Xia, Yun Liu, Dandan Chen, Guibin Pang, Yahui Feng, Huan Yu, Yanxian Wu, Shaodian Zhang, Yangyun Wang, Yong Wang, Hairuo Wen, Leshuai W Zhang
Aim:To explore the hepatotoxicity of copper sulfide nanoparticles (CuSNPs) toward hepatocyte spheroids.Materials & methods:Other than the traditional agarose method to generate hepatocyte spheroids, we developed a multi-concave agarose chip (MCAC) method to investigate changes in hepatocyte viability, morphology, mitochondrial membrane potential, reactive oxygen species and hepatobiliary transporter by CuSNPs.Results:The MCAC method allowed a large number of spheroids to be obtained per sample. CuSNPs showed hepatotoxicityin vitrothrough a decrease in spheroid viability, albumin/urea production and glycogen deposition. CuSNPs also introduced hepatocyte spheroid injury through alteration of mitochondrial membrane potential and reactive oxygen species, that could be reversed by N-acetyl-l-cysteine. CuSNPs significantly decreased the activity of BSEP transporter by downregulating its mRNA and protein levels. Activity of the MRP2 transporter remained unchanged.Conclusion:We observed the hepatotoxicity of CuSNPsin vitrowith associated mechanisms in an advanced 3D culture system. Aim: Materials & methods: Results: in vitro l Conclusion: in vitro.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
Erscheinungsort nicht ermittelbar: 2021 |
| Enthalten in: |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jiang, Tianyan [VerfasserIn] |
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| Links: |
FID Access [lizenzpflichtig] |
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| Themen: |
Copper sulfide nanoparticles |
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| Umfang: |
1 Online-Ressource (18 p) |
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| doi: |
10.2217/nnm-2021-0011 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
KFL011089334 |
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| 100 | 1 | |a Jiang, Tianyan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Hepatotoxicity of copper sulfide nanoparticles towards hepatocyte spheroids using a novel multi-concave agarose chip method |c Tianyan Jiang, Haoxiang Guo, Ya-Nan Xia, Yun Liu, Dandan Chen, Guibin Pang, Yahui Feng, Huan Yu, Yanxian Wu, Shaodian Zhang, Yangyun Wang, Yong Wang, Hairuo Wen, Leshuai W Zhang |
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| 520 | |a Aim:To explore the hepatotoxicity of copper sulfide nanoparticles (CuSNPs) toward hepatocyte spheroids.Materials & methods:Other than the traditional agarose method to generate hepatocyte spheroids, we developed a multi-concave agarose chip (MCAC) method to investigate changes in hepatocyte viability, morphology, mitochondrial membrane potential, reactive oxygen species and hepatobiliary transporter by CuSNPs.Results:The MCAC method allowed a large number of spheroids to be obtained per sample. CuSNPs showed hepatotoxicityin vitrothrough a decrease in spheroid viability, albumin/urea production and glycogen deposition. CuSNPs also introduced hepatocyte spheroid injury through alteration of mitochondrial membrane potential and reactive oxygen species, that could be reversed by N-acetyl-l-cysteine. CuSNPs significantly decreased the activity of BSEP transporter by downregulating its mRNA and protein levels. Activity of the MRP2 transporter remained unchanged.Conclusion:We observed the hepatotoxicity of CuSNPsin vitrowith associated mechanisms in an advanced 3D culture system. Aim: Materials & methods: Results: in vitro l Conclusion: in vitro | ||
| 653 | |a copper sulfide nanoparticles | ||
| 653 | |a hepatobiliary transporters | ||
| 653 | |a hepatotoxicity | ||
| 653 | |a reactive oxygen species | ||
| 653 | |a spheroids | ||
| 700 | 1 | |a Guo, Haoxiang |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Ya-Nan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yun |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Dandan |e verfasserin |4 aut | |
| 700 | 1 | |a Pang, Guibin |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Yahui |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Huan |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Yanxian |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Shaodian |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yangyun |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Wen, Hairuo |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Leshuai W |e verfasserin |4 aut | |
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