Anti-inflammatory and antioxidant properties of unripe papaya extract in an excision wound model / Abdulrazaq Bidemi Nafiu, Mohammad Tariqur Rahman
Abstract Context : Carica papaya L. (Caricaceae) fruit was shown to exhibit wound healing properties. Objectives : We investigated anti-inflammatory and antioxidant potential of papaya fruit phosphate-buffered saline extract (PE) during wound healing and enhancement of the potentials due to trace ions addition. Materials and methods : Rat excision wounds were topically treated twice/day with 20 µL of PE (5 mg extract/mL), 0.5 µg Se 2+ added PE (PES), or 100 µM Zn 2+ added PE (PEZ). Control groups were treated with deionized water (negative) and deproteinized calf blood extract ointment (Solcoseryl®, positive). Lipid peroxidation (LPX), antioxidant, proinflammatory, and arginine metabolic enzymes were estimated in the wound excised on days 4 and 10 post wounding. Results : PE (5 mg/mL; 9.80 ± 0.33 d) and PES (PE + 0.5 µg Se 2+ ; 8.90 ± 0.23 d) significantly ( p < 0.05) reduced the average time for complete wound closure compared with the negative (13.00 ± 0.37 d) and positive (9.80 ± 0.33 d) controls, respectively. Biochemical evaluations of LPX product (malondialdehyde), antioxidant (catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx)), and pro-inflammatory (cyclooxygenase-2 and myeloperoxidase (MPO)) enzyme activities and metabolites (nitrite and urea), on days 4 and 10 post wounding, confirmed the anti-inflammatory and antioxidant properties of PE and PES in this study. Discussion and conclusion : Treatment of excision wounds with papaya extract, especially with the addition of selenium for 10 d, reduced inflammation associated oxidative damage apparently via cyclooxygenase specific inhibition, arginine metabolism, and up-regulation of antioxidant enzymes.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:53 |
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| Enthalten in: |
Pharmaceutical biology - 53(2015), 5, Seite 662- |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Nafiu, Abdulrazaq Bidemi [VerfasserIn] |
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| Links: |
FID Access [lizenzpflichtig] |
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| Umfang: |
1 Online-Ressource (10 p) |
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| doi: |
10.3109/13880209.2014.936470 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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KFL001103563 |
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| 520 | |a Abstract Context : Carica papaya L. (Caricaceae) fruit was shown to exhibit wound healing properties. Objectives : We investigated anti-inflammatory and antioxidant potential of papaya fruit phosphate-buffered saline extract (PE) during wound healing and enhancement of the potentials due to trace ions addition. Materials and methods : Rat excision wounds were topically treated twice/day with 20 µL of PE (5 mg extract/mL), 0.5 µg Se 2+ added PE (PES), or 100 µM Zn 2+ added PE (PEZ). Control groups were treated with deionized water (negative) and deproteinized calf blood extract ointment (Solcoseryl®, positive). Lipid peroxidation (LPX), antioxidant, proinflammatory, and arginine metabolic enzymes were estimated in the wound excised on days 4 and 10 post wounding. Results : PE (5 mg/mL; 9.80 ± 0.33 d) and PES (PE + 0.5 µg Se 2+ ; 8.90 ± 0.23 d) significantly ( p < 0.05) reduced the average time for complete wound closure compared with the negative (13.00 ± 0.37 d) and positive (9.80 ± 0.33 d) controls, respectively. Biochemical evaluations of LPX product (malondialdehyde), antioxidant (catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx)), and pro-inflammatory (cyclooxygenase-2 and myeloperoxidase (MPO)) enzyme activities and metabolites (nitrite and urea), on days 4 and 10 post wounding, confirmed the anti-inflammatory and antioxidant properties of PE and PES in this study. Discussion and conclusion : Treatment of excision wounds with papaya extract, especially with the addition of selenium for 10 d, reduced inflammation associated oxidative damage apparently via cyclooxygenase specific inhibition, arginine metabolism, and up-regulation of antioxidant enzymes | ||
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