Binding of Factors IX and IXato Cultured Vascular Endothelial Cells
Factor IX and its activated form IXahave been found to bind to confluent cultured bovine aortic and human umbilical vein endothelial cells. Binding of bovine factors IX and IXato the bovine endothelial cells was saturable and specific and reached a plateau in 75 min at 4 degrees C and 30 min at 37 degrees C. Binding was half-maximal at a total factor IX or IXaconcentration of 2.3± 0.2 nM. At 4 degrees C, a maximum of 42 fmol of tritiated factor IX or IXabound to 106cells (an average of 20,000 molecules per cell). The binding of tritiated factor IX or IXawas inhibited by excess unlabeled factor IX or IXabut not by factor X, prothrombin, or thrombin. Competition studies indicated that factors IX and IXainteracted with the same site. Binding was reversible, with 50% of the specifically bound factor IX or IXaeluted in 40 min by a 400-fold excess of unlabeled protein. Specific binding required Ca2+with half-maximal binding at 1.2 mM CaCl2. Factor IXabound to the cells was tested for procoagulant activity in a clotting assay with factor IX-deficient plasma, cephalin, and CaCl2. Cell-bound factor IXawas at least 3-fold more active than was factor IXain solution. The retention of procoagulant activity by cell surfacebound factor IXaprovides a mechanism for the localization of clotpromoting activity..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
1983 |
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| Erschienen: |
1983 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:80 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Stern, David M. [VerfasserIn] |
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| Links: |
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| Themen: |
Applied sciences |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
JST070202044 |
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| 035 | |a (JST)14339 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Stern, David M. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Binding of Factors IX and IXato Cultured Vascular Endothelial Cells |
| 264 | 1 | |c 1983 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Factor IX and its activated form IXahave been found to bind to confluent cultured bovine aortic and human umbilical vein endothelial cells. Binding of bovine factors IX and IXato the bovine endothelial cells was saturable and specific and reached a plateau in 75 min at 4 degrees C and 30 min at 37 degrees C. Binding was half-maximal at a total factor IX or IXaconcentration of 2.3± 0.2 nM. At 4 degrees C, a maximum of 42 fmol of tritiated factor IX or IXabound to 106cells (an average of 20,000 molecules per cell). The binding of tritiated factor IX or IXawas inhibited by excess unlabeled factor IX or IXabut not by factor X, prothrombin, or thrombin. Competition studies indicated that factors IX and IXainteracted with the same site. Binding was reversible, with 50% of the specifically bound factor IX or IXaeluted in 40 min by a 400-fold excess of unlabeled protein. Specific binding required Ca2+with half-maximal binding at 1.2 mM CaCl2. Factor IXabound to the cells was tested for procoagulant activity in a clotting assay with factor IX-deficient plasma, cephalin, and CaCl2. Cell-bound factor IXawas at least 3-fold more active than was factor IXain solution. The retention of procoagulant activity by cell surfacebound factor IXaprovides a mechanism for the localization of clotpromoting activity. | ||
| 650 | 4 | |a Medical Sciences | |
| 650 | 4 | |a Hemostasis | |
| 650 | 4 | |a Thrombosis | |
| 650 | 4 | |a Clot Localization | |
| 650 | 4 | |a Intrinsic System | |
| 650 | 4 | |a Biological sciences |x Biology |x Zoology |x Animals |x Mammals |x Ungulates | |
| 650 | 4 | |a Biological sciences |x Biology |x Cytology |x Cell biology |x Cells |x Epithelial cells |x Endothelial cells | |
| 650 | 4 | |a Health sciences |x Medical sciences |x Pharmaceutics |x Pharmaceutical preparations |x Medications |x Coagulants | |
| 650 | 4 | |a Physical sciences |x Physics |x Microphysics |x Nuclear physics |x Nuclear reactions |x Radioactive decay | |
| 650 | 4 | |a Applied sciences |x Materials science |x Materials |x Gels | |
| 650 | 4 | |a Biological sciences |x Biology |x Physiology |x System physiology |x Cardiovascular physiology |x Blood circulation |x Hemostasis |x Blood coagulation |x Clotting | |
| 650 | 4 | |a Biological sciences |x Biology |x Cytology |x Cell biology |x Cells |x Cultured cells | |
| 650 | 4 | |a Physical sciences |x Chemistry |x Chemical compounds |x Chemicals |x Ligands | |
| 650 | 4 | |a Physical sciences |x Chemistry |x Chemical elements |x Alkaline earth metals |x Calcium | |
| 650 | 4 | |a Biological sciences |x Biology |x Cytology |x Cell biology |x Cells |x Cultured cells |x Cell lines |x Human umbilical vein endothelial cells | |
| 655 | 4 | |a research-article | |
| 700 | 1 | |a Drillings, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Nossel, Hymie L. |e verfasserin |4 aut | |
| 700 | 1 | |a Hurlet-Jensen, Anne |e verfasserin |4 aut | |
| 700 | 1 | |a LaGamma, Kalliope S. |e verfasserin |4 aut | |
| 700 | 1 | |a Owen, John |e verfasserin |4 aut | |
| 773 | 1 | 8 | |g volume:80 |g year:1983 |g number:13 |g pages:4119-4123 |
| 856 | 4 | 0 | |u https://www.jstor.org/stable/14339 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_JST | ||
| 951 | |a AR | ||
| 952 | |d 80 |j 1983 |e 13 |h 4119-4123 | ||